PMID: 8944705Nov 1, 1996Paper

Relationship between polyamines, actin distribution, and gastric healing in rats

The American Journal of Physiology
A BananL R Johnson

Abstract

Intragastric administration of 3.4 M NaCl damages the gastric mucosa and increases the activity of ornithine decarboxylase (ODC), the rate-limiting enzyme in polyamine synthesis. Polyamines are essential for the repair of gastric erosions. Little is known about the restitution of damaged mucosa except that cell migration is essential. Actin is the principal cytoskeletal protein and is essential for migration. This investigation determines the relationship between polyamines, actin, and gastric healing. Rats were fasted for 22 h and given 1.0 ml of 3.4 M NaCl intragastrically and killed 1, 2, 4, 8, and 10 h later. The mucosa was assayed for ODC activity and stained for G- and F-actin. F-actin was concentrated below the damaged mucosa at 1.5, 2, and 4 h. There was no increase in F-actin distribution at any time point, when NaCl-treated animals were given alpha-difluoromethylornithine (DFMO), a specific inhibitor of ODC. In addition, DFMO significantly prevented the healing of the mucosal lesions. Spermidine treatment after DFMO + NaCl significantly prevented the effects of DFMO. Cytochalasin D, a potent actin-disrupting drug, significantly delayed normal gastric mucosal healing. The endogenous polyamines increased significantly i...Continue Reading

Citations

Apr 9, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·A BananA Keshavarzian
Apr 19, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·A BananA Keshavarzian
Jun 8, 2001·News in Physiological Sciences : an International Journal of Physiology Produced Jointly by the International Union of Physiological Sciences and the American Physiological Society·Leonard R. Johnson, Shirley A. McCormack
Sep 12, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·A BananA Keshavarzian
May 16, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·A BananA Keshavarzian
Jul 12, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·A BananA Keshavarzian
Apr 13, 2007·Physiological Reviews·Anthony T BlikslagerJack Odle
Aug 4, 2018·Journal of Cell Science·Eitaro AiharaMarshall H Montrose

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