Relationship between protein and mitochondrial DNA oxidative injury and telomere length and muscle loss in healthy elderly subjects

Archives of Gerontology and Geriatrics
Daniel BunoutSandra Hirsch

Abstract

A blood sample and muscle biopsies were obtained from 54 elderly subjects. Twenty-seven subjects aged 77+/-3 years, had experienced a change in fat free mass (FFM) of +194+/-282g/year (lean body mass maintainers) and 27 subjects aged 78+/-3 years, had a change in FFM of -487+/-209g/year (lean body mass losers). Muscle biopsies were also obtained from 10 healthy subjects aged 34+/-4 years. In muscle, the ratio of mitochondrial DNA (mtDNA) to nuclear DNA (nDNA) and telomere length were assessed and deposition of 4-hydroxy-2-nonenal adducts (4HNE) was visualized by electron microscopy. In FFM maintainers, losers and young controls, the ratio of mtDNA to nDNA was 2.1 (95% confidence intervals (CI), 0.1-31.7), 1.5 (95% CI, 0.2-15.7) and 18.6 (95% CI, 2.8-46.2), respectively. 4HNE deposition was 5.9 (95% CI, 1.5-28), 4.9 (95% CI, 0.9-13) and 3.4 (95% CI, 1.1-4.6) gold particles/microm(2), respectively. Telomere length, expressed as T/S ratio, was 0.06 (95% CI, 0.01-0.16), 0.06 (95% CI, 0.03-0.27) and 0.34 (95% CI, 0.1-1.34), respectively (p<0.02 or less for all comparisons between elderly and young subjects).

References

Aug 1, 1991·Medicine and Science in Sports and Exercise·L E VoorripsW A Van Staveren
Jan 12, 2000·Toxicological Sciences : an Official Journal of the Society of Toxicology·B C HansenH K Ortmeyer
Mar 14, 2000·Neuromuscular Disorders : NMD·S DecaryG S Butler-Browne
Sep 7, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·T A ZainalR Weindruch
May 10, 2002·Nucleic Acids Research·Richard M Cawthon
Nov 2, 2002·Journal of the American Geriatrics Society·Kirsten Krahnstoever DavisonWilliam H Dietz
Dec 11, 2002·Journal of the American Geriatrics Society·Luigi FerrucciJack M Guralnik Md
Dec 24, 2002·Journal of Applied Physiology·Stephen WelleCharles A Thornton
Sep 16, 2003·Medicine and Science in Sports and Exercise·Malcolm CollinsVincent Mouly
Dec 4, 2003·Science·Irina M ConboyThomas A Rando
May 5, 2004·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Giorgio ValentiLuigi Ferrucci
May 6, 2004·Annals of the New York Academy of Sciences·Ren-Kui BaiLee-Jun C Wong
Jul 29, 2004·Clinical Science·Mikhail F AlexeyevGlenn L Wilson
Nov 13, 2004·Hormone Research·Annice MukherjeeStephen M Shalet
Jan 5, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Anne GarnierRenée Ventura-Clapier
Apr 1, 2005·Proceedings of the National Academy of Sciences of the United States of America·Kevin R ShortK Sreekumaran Nair
May 18, 2005·Biochemical and Biophysical Research Communications·Béatrice ChabiGeorges Stepien
Aug 16, 2005·Mechanisms of Ageing and Development·Thomas FrahmChristoph Meissner
May 12, 2006·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·María-Pía de la MazaMireya Fernández
Jun 27, 2006·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Elizabeth V MenshikovaBret H Goodpaster
Nov 8, 2006·The Journal of Endocrinology·A M Solomon, P M G Bouloux
Aug 30, 2007·Aging Clinical and Experimental Research·Daniel BunoutSandra Hirsch

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Citations

Jul 14, 2011·Photomedicine and Laser Surgery·Fernanda Rossi PaolilloVanderlei Salvador Bagnato
Mar 16, 2011·Journal of Aging Research·Andrew T Ludlow, Stephen M Roth
Oct 8, 2014·Geriatric Nursing·Gladys BarreraSandra Hirsch
Jun 16, 2011·Australasian Journal on Ageing·Daniel BunoutSandra Hirsch
Mar 26, 2014·Journal of Human Nutrition and Dietetics : the Official Journal of the British Dietetic Association·D BunoutS Hirsch
Mar 27, 2015·The Journal of Nutrition, Health & Aging·M P de la MazaD Bunout
Jun 16, 2012·Clinical Nursing Research·Barbara ResnickChris Wells
Mar 25, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Larissa Alexsandra da Silva Neto TrajanoAdenilson de Souza da Fonseca

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