Relationship between protein stability and functional activity in the presence of macromolecular crowding agents alone and in mixture: An insight into stability-activity trade-off

Archives of Biochemistry and Biophysics
Sumra ShahidAsimul Islam

Abstract

The cellular environment is crowded with different kinds of molecules with varying sizes, shapes and compositions. Most of the experiments studying the nature and behaviour of a protein have been done on the isolated protein in dilute buffer solutions which actually do not imitate the in vivo situation. To understand the consequences of such crowded environment, we investigated the effect of macromolecular crowding on the stability and activity of hen egg white lysozyme. Two crowding agents, dextran 70 and ficoll 70 which have different shapes and composition, have been employed in this study. To mimic the cellular condition from physiological point of view, the effect of mixtures of both the crowding agents has been also studied. The results indicate that owing to volume exclusion, lysozyme is stabilized while its activity decays with the increasing concentration of both the crowders elucidating the hypothesis of stability-activity trade-off. Mixed macromolecular crowding exerts greater effect than the sum of constituent crowding agents (dextran 70 and ficoll 70).

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Citations

Mar 1, 2017·International Journal of Biological Macromolecules·Zahoor Ahmad ParrayAsimul Islam
Oct 4, 2017·Protein Science : a Publication of the Protein Society·Luis C AcostaAnnelise H Gorensek-Benitez
Feb 10, 2019·Applied Biochemistry and Biotechnology·Sumra ShahidAsimul Islam
May 8, 2020·Analytical and Bioanalytical Chemistry·Rouba NasreddineReine Nehmé
Jul 17, 2020·International Journal of Molecular Sciences·Natalia A ChebotarevaBoris I Kurganov
Nov 18, 2020·International Journal of Molecular Sciences·Murial L RossPrashanth Asuri
Mar 6, 2021·Biochemistry and Biophysics Reports·Xander E WilcoxKristin M Slade
May 14, 2021·The Journal of Physical Chemistry. B·Zikri HasanbasriSunil Saxena
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zahoor Ahmad ParrayAsimul Islam
Jul 20, 2018·ACS Omega·Saikat BiswasPramit Kumar Chowdhury

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