PMID: 2106136Feb 1, 1990Paper

Relationship between protein synthesis and concentrations of charged and uncharged tRNATrp in Escherichia coli

Proceedings of the National Academy of Sciences of the United States of America
M V RojianiE Goldman

Abstract

We have continuously monitored Trp-tRNA(Trp) concentrations in vivo and, in the same cultures, measured rates of protein synthesis in isogenic stringent and relaxed strains. We have also manipulated cellular charged and uncharged [tRNA(Trp)] by two means: (i) the strain used contains a Trp-tRNA synthetase mutation that increases the Km for Trp; thus, varying exogenous Trp varies cellular Trp-tRNA(Trp); and (ii) we have introduced into the mutant strain a plasmid containing the tRNA(Trp) gene behind an inducible promoter; thus, total [tRNA(Trp)] also can be varied depending on length of induction. The use of these conditions, combined with a previously characterized assay system, has allowed us to demonstrate that (i) the rate of incorporation of Trp into protein is proportional to the fraction of tRNA(Trp) that is charged; for any given total [tRNA(Trp)], this rate is also proportional to the [Trp-tRNA(Trp)]; (ii) uncharged tRNA(Trp) inhibits incorporation of Trp into protein; and (iii) rates of incorporation into protein of at least two other amino acids, Lys and Cys, are also sensitive to [Trp-tRNA(Trp)] and are inhibited by uncharged tRNA(Trp). Our results are consistent with models of translational control that postulate mo...Continue Reading

References

Jan 1, 1979·Annual Review of Genetics·J A Gallant
May 20, 1989·Journal of Molecular Biology·M A SørensenS Pedersen
Sep 5, 1989·Journal of Molecular Biology·J F Curran, M Yarus
Dec 1, 1986·Molecular & General Genetics : MGG·A K Ulrich, J Parker
May 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·W A Haseltine, R Block
Feb 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·W F Anderson
Sep 11, 1984·Nucleic Acids Research·M RobinsonG Humphreys
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·W E Holben, E A Morgan
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·H A de BoerM Vasser
Dec 25, 1983·Journal of Molecular Biology·J R Menninger
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·L W DeBoerE Braunwald
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·H J RheinbergerK H Nierhaus
Jan 10, 1981·Nucleic Acids Research·R GranthamR Mercier

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Citations

Mar 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J Sipley, E Goldman
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·J A Enríquez, G Attardi
Aug 5, 2000·Applied and Environmental Microbiology·A G Zdanovsky, M V Zdanovskaia
Sep 18, 2009·PloS One·Mark WelchClaes Gustafsson
Mar 20, 2012·Protein Expression and Purification·Claes GustafssonMark Welch
Mar 16, 2017·Trends in Genetics : TIG·Christina E Brule, Elizabeth J Grayhack
Apr 7, 2018·FEBS Letters·Charlotte MignonBettina Werle
Dec 1, 1990·Molecular Microbiology·E Goldman, H Jakubowski
Jan 1, 1993·Molecular Microbiology·S Z WahabW M Holmes
Oct 27, 1997·The Journal of Biological Chemistry·A FlierlP Seibel
Aug 3, 2004·Journal of Cell Science·Sang Won LeeSunghoon Kim
Dec 1, 1995·Microbiological Reviews·C CondonC L Squires
Jun 1, 1992·Journal of Bacteriology·P G JonesF C Neidhardt
Apr 1, 1997·Journal of Bacteriology·U Mechold, H Malke

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