Relationship between serum HBsAg level, HBV DNA level, and peripheral immune cells in patients with chronic hepatitis B virus infection

Hepatic Medicine : Evidence and Research
Rm MukherjeeDn Reddy

Abstract

The chronicity of hepatitis B virus (HBV) infection is attributed to inappropriate functioning of cell-mediated immunity. Besides the importance of measuring serum HBV DNA and HBV surface antigen (HBsAg) as markers of viral replication and exposure, respectively, studies regarding their influence on immune cell status in chronic HBV infection are still scarce. Because such studies of chronic HBV patients have not been reported for India, we attempted to evaluate the relationship between serum concentrations of HBsAg, HBV DNA, and percentage of immune cells in peripheral blood of Indian subjects with chronic HBV infection. Thirty-one HbsAg-positive subjects were evaluated for serum HBe antigen (HBeAg), anti-HBe, and alanine transferase status by standard enzyme-linked immunosorbent assay (ELISA) and biochemical procedures. Serum HBV DNA level was determined by real-time TaqMan® polymerase chain reaction assay. Serum HBsAg level was measured by a third-generation sandwich ELISA kit. Peripheral immune cell profiling was done by multifluorometric flow cytometry analysis, for which 21 healthy subjects were included as controls. The majority (93.5%) of the study subjects were HBeAg-negative and anti-HBeAg-positive. Mean viral load, H...Continue Reading

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA
fluorescence-activated cell sorter

Software Mentioned

SPSS

Related Concepts

Related Feeds

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.

Related Papers

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
S D LeeB N Chiang
Revista de medicină internă, neurologe, psihiatrie, neurochirurgie, dermato-venerologie. Medicină internă
L StanciuS Popescu
Bacteriologia, virusologia, parazitologia, epidemiologia
C ClaiciE Don
Nihon Shokakibyo Gakkai zasshi = The Japanese journal of gastro-enterology
T SakataO Wada
© 2021 Meta ULC. All rights reserved