PMID: 9168147Apr 26, 1997Paper

Relationship between specific binding of 125I-omega-conotoxin GVIA and GTP binding protein: effects of the GTP analogues, mastoparan and A1F4-

Biochimica Et Biophysica Acta
Seiji IchidaY A Zhang

Abstract

We investigated whether the specific binding or labeling of 125I-omega-CgTX on crude membranes from chick whole brain was affected when endogenous GTP binding protein (G protein) was activated by GTP analogues, mastoparan (MP) and aluminum fluoride (AIF4-; AICl3 + NaF). Both GTPgammaS and Gpp(NH)p attenuated the inhibitory effect of selective N-type Ca channel inhibitors such as aminoglycoside antibiotics (AGs) or dynorphine (1-13)(Dyn) on specific 125I-omega-CgTX binding in a dose-dependent manner. On the other hand, the inhibitory effects of the divalent metal cations Cd2+, Co2+, Mg2+ and Mn2- on such binding were not attenuated by GTPgammaS. MP and AIF4- also attenuated the inhibitory effect of Neo on this binding similar to GTPgammaS. The attenuating effect of MP was enhanced by the presence of Mg2+ in a dose-dependent manner. However, GTP analogues, MP and AIF4-, did not affect binding or labeling without AGs or Dyn. GTPgammaS, MP and AIF4- also attenuated the specific labeling of a 215-kDa band in crude membranes with 125I-omega-CgTX using the cross-linker DSS (non-reduced condition) in the presence of Neo. These results indicate that there are direct or indirect relationships between N-type Ca channels and G proteins via...Continue Reading

References

Feb 15, 1988·Biochemical and Biophysical Research Communications·J BarhaninM Lazdunski
Jan 1, 1988·Cold Spring Harbor Symposia on Quantitative Biology·E J NeerD Kim
Jan 1, 1989·Annals of the New York Academy of Sciences·H GlossmannH G Schindler
Jan 1, 1989·Progress in Biophysics and Molecular Biology·E Carbone, D Swandulla
Jan 1, 1988·Cold Spring Harbor Symposia on Quantitative Biology·M ChabreT M Vuong

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