Relationship between sperm apoptosis and bull fertility: in vivo and in vitro studies

Reproduction, Fertility, and Development
Lauren EricksonMuhammad Anzar

Abstract

The objectives of this study were to confirm the relationship of apoptosis-associated membrane and nuclear changes in bull spermatozoa with field fertility, to predict the fertility of beef bulls used for natural breeding and to study the role of DNA-nicked spermatozoa in early embryonic development. In Experiment 1, the relationship between fertility and different sperm populations identified by the Annexin V/propidium iodide (PI) and terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) assays was determined. Bull fertility was related to live (PPPin vitro cleavage and blastocyst rates was evaluated, using 30000 or 300000 spermatozoa per droplet. Cleavage rate was adversely affected (PP<0.05) in high DNA-nicked spermatozoa at the lower sperm concentration. In conclusion, the incidence of DNA-nicked spermatozoa is a useful marker to predict a bull's fertility potential. DNA-nicked spermatozoa showed adverse effects on early embryonic development.

References

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Citations

Mar 8, 2016·Animal Reproduction Science·J RocaH Rodriguez-Martinez
Dec 7, 2019·Journal of Animal Science and Biotechnology·MariaPortia B NagataKenichi Yamashita
Mar 21, 2018·Proceedings of the National Academy of Sciences of the United States of America·Maria Portia B NagataKenichi Yamashita

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Methods Mentioned

BETA
electrophoresis

Software Mentioned

FlowMax
BD CellQuest Pro
SPSS

Related Concepts

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis