Abstract
The effects of thallous cation (Tl(+)) on: (a) the production of oxidant species and (b) membrane fluidity were evaluated in human leukemia T cells (Jurkat). After 72 h of incubation in the presence of Tl(+) (5-100 microM), no significant changes in cell viability were observed, although the average cell size was decreased as evaluated by steady-state light scattering. Tl(+) (5-100 microM) caused a significant increase in the concentration of cellular oxidants as measured with the probe 5(6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate (DCDCDHF). Similarly, a higher lipid oxidation products release was observed as measured by TBARS production. Both Tl(+)-mediated DCDCDHF oxidation and TBARS production were prevented when cells were supplemented with 2mM Trolox. Tl(+) (5-100 microM) also induced a concentration-dependent increase in plasma membrane fluidity, evaluated with the probe 6-(9-anthroyloxy)stearic acid (6-AS). This effect of Tl(+) was neither associated to the externalization of phosphatidylserine, nor observed in Trolox-supplemented cells. Significant correlations were found between the increase in plasma membrane fluidity and TBARS production and DCDCDHF oxidation. Together, the present results suggest that the...Continue Reading
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