Relationship between thallium(I)-mediated plasma membrane fluidification and cell oxidants production in Jurkat T cells

Toxicology
Sandra V Verstraeten

Abstract

The effects of thallous cation (Tl(+)) on: (a) the production of oxidant species and (b) membrane fluidity were evaluated in human leukemia T cells (Jurkat). After 72 h of incubation in the presence of Tl(+) (5-100 microM), no significant changes in cell viability were observed, although the average cell size was decreased as evaluated by steady-state light scattering. Tl(+) (5-100 microM) caused a significant increase in the concentration of cellular oxidants as measured with the probe 5(6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate (DCDCDHF). Similarly, a higher lipid oxidation products release was observed as measured by TBARS production. Both Tl(+)-mediated DCDCDHF oxidation and TBARS production were prevented when cells were supplemented with 2mM Trolox. Tl(+) (5-100 microM) also induced a concentration-dependent increase in plasma membrane fluidity, evaluated with the probe 6-(9-anthroyloxy)stearic acid (6-AS). This effect of Tl(+) was neither associated to the externalization of phosphatidylserine, nor observed in Trolox-supplemented cells. Significant correlations were found between the increase in plasma membrane fluidity and TBARS production and DCDCDHF oxidation. Together, the present results suggest that the...Continue Reading

References

Jan 1, 1990·The International Journal of Biochemistry·K T DouglasS R Baindur
May 31, 1994·Toxicology·S Galván-Arzate, C Ríos
Jun 5, 1993·BMJ : British Medical Journal·D MooreA Dixon
Nov 4, 1998·Toxicology Letters·S Galván-Arzate, A Santamaría
May 18, 1999·Biochemical and Biophysical Research Communications·M L TomassoniM V Magni
Jan 6, 2000·Archives of Biochemistry and Biophysics·A AroraG M Strasburg
Feb 28, 2002·Journal of Immunological Methods·Tonya LaakkoPam Fraker
Dec 5, 2002·Archives of Biochemistry and Biophysics·Sandra V Verstraeten, Patricia I Oteiza
Dec 31, 2002·Journal of Receptor and Signal Transduction Research·Jean-Michel MaixentSamuel Lévy
Aug 28, 2003·Archives of Biochemistry and Biophysics·Marcela S Villaverde, Sandra V Verstraeten
Oct 29, 2003·Toxicological Reviews·Robert S Hoffman
Mar 23, 2004·Annals of the New York Academy of Sciences·Marcantonio BragadinMarcella Canton
Dec 29, 2004·Free Radical Research·Marcela S VillaverdeSandra V Verstraeten

❮ Previous
Next ❯

Citations

Sep 7, 2007·Environmental Health Perspectives·Herbert RiechelmannRudolf Reiter
Mar 23, 2011·Journal of Bioenergetics and Biomembranes·Sergey M Korotkov, Nils-Erik L Saris
Sep 14, 2012·Drug and Chemical Toxicology·Juan José Rodríguez-Mercado, Mario Agustín Altamirano-Lozano
Jun 16, 2010·Environmental Toxicology·Jalal PourahmadBahram Daraei
Dec 10, 2013·Environmental Toxicology·Juan J Rodríguez-MercadoMario A Altamirano-Lozano
Dec 25, 2010·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·C NavarroM Bermejo
Jan 29, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Gustavo CélizSandra V Verstraeten
Jan 5, 2013·Toxicology·Xavier TekpliDominique Lagadic-Gossmann
Jul 15, 2016·Archives of Toxicology·María Teresa Luján PinoSandra Viviana Verstraeten
May 30, 2020·Neurotoxicity Research·María Ester Hurtado-DíazAbel Santamaría

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis