Relationship of 16S rRNA sequence similarity to DNA hybridization in prokaryotes

International Journal of Systematic and Evolutionary Microbiology
J Keswani, W B Whitman

Abstract

The relationship between 16S rRNA sequence similarity (S) and the extent of DNA hybridization (D) was well described by the equation In(-InD) = 0.53 [In(-InS)]+2.201 when D was determined by either the S1 nuclease or membrane filter methods. When the presence of nonultrametric rRNA sequences and differences between genera or families were controlled, this relationship accounted for 78% of the variability of D given S, and it was possible to estimate the distribution of D from S with a known precision. Thus, D<0.70 was expected to occur 50, 95 and 99% of the time when S was 0.998, 0.992 and 0.986, respectively. The relationship between D and S varied between prokaryotic taxa even within the same subphylum, and more precise estimates of D could be made when the relationship for a particular taxon was known. The relationship between D and S was not significantly different between the prokaryotic domains, and S appeared to be a quasi-molecular clock of approximately constant rate when averaging effects and stochastic factors were taken into account. The relationship between logD and logS was nonlinear, and D provided a very poor measure of relatedness for distantly related organisms. For instance, within the range 1.0 >S> 0.95, D d...Continue Reading

Citations

May 12, 2010·Forensic Science International. Genetics·Bhawna DubeyIkramul Haque
Aug 4, 2015·International Journal of Systematic and Evolutionary Microbiology·Liang GeLizhe An
Dec 26, 2015·International Journal of Systematic and Evolutionary Microbiology·Seil KimYoungsoon Um
Jan 11, 2014·International Journal of Systematic and Evolutionary Microbiology·Yong-Fu LiEmily Bulian Helmes
Jun 19, 2013·International Journal of Systematic and Evolutionary Microbiology·Jong-Hwan LeeByoung-Chan Kim
Jul 2, 2009·International Journal of Systematic and Evolutionary Microbiology·Marilize Le Roes-HillStephanie G Burton
Feb 8, 2014·International Journal of Systematic and Evolutionary Microbiology·Mincheol KimJongsik Chun
Dec 9, 2008·International Journal of Systematic and Evolutionary Microbiology·Dong-Shan AnMin-Ho Yoon
May 9, 2012·International Journal of Systematic and Evolutionary Microbiology·Kohei NakamuraYoichi Kamagata
Aug 3, 2013·International Journal of Systematic and Evolutionary Microbiology·Raika WiertzAndré Lipski
Jun 30, 2010·International Journal of Systematic and Evolutionary Microbiology·Qi ZhaoLizhe An
Aug 31, 2013·International Journal of Systematic and Evolutionary Microbiology·Katsuhiko FujiiYuya Enokida
Dec 14, 2011·International Journal of Systematic and Evolutionary Microbiology·André Lipski, Peter Kämpfer
Mar 31, 2009·International Journal of Systematic and Evolutionary Microbiology·Hiroyuki ImachiKen Takai
May 2, 2009·International Journal of Systematic and Evolutionary Microbiology·Tianfeng TanZongze Shao
Aug 12, 2009·International Journal of Systematic and Evolutionary Microbiology·Yuto YashiroHiroyuki Imachi
Jan 16, 2014·International Journal of Systematic and Evolutionary Microbiology·Julia DerichsAndré Lipski
Oct 20, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·J C Garcia-BetancurM J Garcia
Dec 2, 2011·Annual Review of Food Science and Technology·Sarah O'Flaherty, Todd R Klaenhammer
Oct 4, 2016·International Journal of Systematic and Evolutionary Microbiology·Hideyuki KouzuiKatsuhiko Fujii
Mar 15, 2018·International Journal of Systematic and Evolutionary Microbiology·Chitti Thawai
May 14, 2008·Nature Reviews. Microbiology·Duccio MediniRino Rappuoli
Dec 15, 2010·Applied and Environmental Microbiology·Sue-Yao Wu, Mei-Chin Lai
May 20, 2014·Applied and Environmental Microbiology·David W WaiteMichael W Taylor
Jul 29, 2006·Environmental Microbiology·Frank MaixnerHolger Daims
Feb 24, 2005·Antonie van Leeuwenhoek·James E M Stach, Alan T Bull
Sep 23, 2008·Antonie van Leeuwenhoek·Gareth J Everest, Paul R Meyers
Feb 4, 2010·The Journal of Microbiology·Shengwen DuanLifeng Cheng
Jan 20, 2006·FEMS Microbiology Ecology·Andrew BissettChris Burke
Oct 24, 2008·Environmental Technology·S M Tiquia
Dec 7, 2013·Antonie van Leeuwenhoek·Aharon Oren, George M Garrity
Jun 15, 2011·Antonie van Leeuwenhoek·Gareth J EverestPaul R Meyers
Aug 15, 2014·Nature Reviews. Microbiology·Pablo YarzaRamon Rosselló-Móra

❮ Previous
Next ❯

Related Concepts

Related Feeds

Archaeogenetics

Recent advances in genomic sequencing has led to the discovery of new strains of Archaea and shed light on their evolutionary history. Discover the latest research on Archaeogenetics here.