Relationship of actin filament assembly to clearance of fibrinogen gold, GPIIb-IIIa complexes on spread platelets

European Journal of Clinical Investigation
J G WhiteC M Smith

Abstract

The present study has evaluated the influence of high concentrations of cytochalasins B and E on the detergent-resistant actin levels in fully spread platelets by PAGE gel electrophoresis, and the effects of the two inhibitors of new actin filament assembly on translocation of fibrinogen gold (Fgn/Au) labelled GPIIb-IIIa receptors on the surface-activated cells. Concentrations of 10(-4) M and 10(-5) M cytochalasin B and E reduced detergent-resistant actin in fully spread platelets to levels present in resting discoid platelets in suspension. Despite reduction of actin filaments to levels in resting cells, cytochalasin B did not prevent translocation of Fgn/Au from platelet margins into channels of the open canalicular system (OCS). Similar concentrations of cytochalasin E completely blocked translocation of receptor-ligand complexes and produced a patching phenomenon not observed in previous studies. Rinsing of the spread cells to remove cytochalasin, followed by incubation of the treated platelets in Hank's buffered salt solution (HBSS) restored levels of detergent-resistant actin to those found in untreated, spread platelets. Resting grids of 10(-5) M cytochalasin E-treated platelets on drops of HBSS for 15 min restored their...Continue Reading

References

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Citations

Jul 1, 1995·British Journal of Haematology·J G WhiteG Escolar
Jun 1, 1997·The Journal of Laboratory and Clinical Medicine·J G White
Jul 1, 1996·Arteriosclerosis, Thrombosis, and Vascular Biology·J G WhiteG Escolar
Aug 29, 2003·Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie·Evan G PivalizzaRalf Gebhard
Jul 18, 2009·Annals of Hematology·Massimo FranchiniGiuseppe Lippi
Jul 16, 2014·Nature Reviews. Gastroenterology & Hepatology·Neeral L ShahStephen H Caldwell
Jan 7, 2003·Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie·Andrew Y C WongEdmond S K Ma

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