Relationship of brown adipose tissue perfusion and function: a study through β2-adrenoreceptor stimulation

Journal of Applied Physiology
Laura ErnandeMarielle Scherrer-Crosbie

Abstract

Brown adipose tissue (BAT) activation increases glucose and lipid consumption; as such, it is been considered as a potential therapy to decrease obesity. BAT is highly vascularized and its activation is associated with a necessary increase in blood flow. However, whether increasing BAT blood flow per se increases BAT activity is unknown. To examine this hypothesis, we investigated whether an isolated increase in BAT blood flow obtained by β2-adrenoreceptor (β2-AR) stimulation with salbutamol increased BAT activity. BAT blood flow was estimated in vivo in mice using contrast-enhanced ultrasound. The absence of direct effect of salbutamol on the function of isolated brown adipocytes was assessed by measuring oxygen consumption. The effect of salbutamol on BAT activity was investigated by measuring BAT glucose uptake in vivo. BAT blood flow increased by 2.3 ± 0.6-fold during β2-AR stimulation using salbutamol infusion in mice (P= 0.003). β2-AR gene expression was detectable in BAT but was extremely low in isolated brown adipocytes. Oxygen consumption of isolated brown adipocytes did not change with salbutamol exposure, confirming the absence of a direct effect of β2-AR agonist on brown adipocytes. Finally, β2-AR stimulation by sal...Continue Reading

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Citations

Feb 26, 2019·British Journal of Pharmacology·Bronwyn A EvansDana S Hutchinson
Apr 10, 2020·The Biochemical Journal·Marie Anne RichardDenis P Blondin
Oct 4, 2020·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Gabriel RichardMartin Lepage
Aug 28, 2020·Frontiers in Endocrinology·Mingming WuDimitrios C Karampinos
Jun 5, 2019·Nature Metabolism·Lawrence KazakBruce M Spiegelman

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