Relationship of cell surface hydrophobicity with biofilm formation and growth rate: A study on Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli

Iranian Journal of Basic Medical Sciences
Zulfiqar Ali MiraniTanveer Abbas

Abstract

This study was designed to determine the relationship of Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli isolates in multispecies biofilms and their individual phenotypic characters in biofilm consortia. The subject isolates were recovered from different food samples and identified on the basis of growth on differential and selective media. Tube methods, Congo-red agar method, and scanning electron microscopy (SEM) were used to study biofilms phenotypes. The hydrophobicity of the strains was evaluated by the adhesion to apolar solvent. The results showed that E. coli dominated the pre-biofilm stage. It has been observed that E. coli adopted biofilm life much before S. aureus and P. aeruginosa. However, after adopting biofilm lifestyle, slowly and gradually, P. aeruginosa dominated the consortia and dispersed other stakeholders. The subject isolates of P. aeruginosa produce cis-2-decanoic acid to disperse or inhibit S. aureus and E. coli biofilms. Gas-chromatography and mass spectrometry results showed that cis-2-decanoic was higher in the co-culture condition and increased at late log-phase or at stationary phase. Although majority of S. aureus were unable to compete with P. aeruginosa, however, a minor popu...Continue Reading

Citations

Aug 6, 2020·International Journal of Molecular Sciences·Jana Al AzzazPierre Lapaquette
Jan 14, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nitin Chandra Teja DadiHelena Bujdáková
Jun 23, 2020·Biomacromolecules·Christoph FedererAndreas Bernkop-Schnürch

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