PMID: 7016542Apr 1, 1981Paper

Relationship of microtubule organization in lymphocyte to the capping of immunoglobulin

European Journal of Cell Biology
K A RogersD L Brown

Abstract

We have used a double fluorescence staining procedure to examine the distribution of antitubulin-staining structures in mouse splenic lymphocytes induced to patch and cap surface immunoglobulin by treatment with antiimmunoglobulin antibodies. A well organized network of fibers, extending from a single microtubule organizing center (MTOC) associated with the centriole pair, was detected by antitubulin staining at all stages of the capping process. In lymphocytes possessing as intact network, the cap was formed and internalized over the region of the cytoplasm containing the organizing center, Golgi apparatus, and most other organelles. In contrast, in lymphocytes which had been subjected to a colchicine treatment sufficient to completely disassemble networks prior to cap induction, no relationship was detected by immunofluorescence between the position of the centrioles (a marker for the MTOC) and the location of the surface cap. Electron microscopic observation of colchicine-treated cells, induced to cap by ferritin-conjugated anti-Ig, revealed extensive disorganization of cytoplasmic organelles and exclusion of organelles from the region of underlying the cap. These results indicate a role for the microtubule network of lympho...Continue Reading

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.