Relationship of retinal configuration and internal proton transfer at the end of the bacteriorhodopsin photocycle

Biochemistry
H T RichterJ K Lanyi

Abstract

In the last step of the bacteriorhodopsin photocycle the initial state is regenerated from the O intermediate in an essentially unidirectional reaction. Comparison of the rate of this photocycle step and the rate of deprotonation of Asp-85 in pH jump experiments with various site-specific mutants indicates that recovery of the initial state is influenced by (1) residues such as Glu-204 that affect deprotonation of Asp-85 and (2) residues such as Leu-93 that contact the retinal and therefore must affect its thermal reisomerization from 13-cis to all-trans as suggested by Delaney, Schweiger, and Subramaniam (Proc. Natl. Acad. Sci. U.S.A. 92, 11120-11124, 1995). These results, together with FTIR spectra (Kandori, Hatanaka, Yamazaki, Needleman, Brown, Richter, Lanyi, & Maeda, manuscript in preparation) of the last intermediate in the photocycles of representatives of the two kinds of mutants, E204Q and L93M, suggest the following sequence of events: reisomerization of the retinal from 13-cis to an all-trans configuration that contains a twisted chain (with high amplitude hydrogen out-of-plane vibrational bands) triggers proton transfer from Asp-85 to Glu-204 or directly to the extracellular surface, and the proton transfer in turn ...Continue Reading

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Citations

May 2, 2009·Journal of the American Chemical Society·Prasad PhatakMarcus Elstner
Oct 23, 2009·Photochemistry and Photobiology·Li-Kang Chu, Mostafa A El-Sayed
Feb 24, 2004·Annual Review of Physiology·Janos K Lanyi
Jun 26, 2007·Journal of Molecular Microbiology and Biotechnology·Janos K Lanyi
Nov 5, 2015·Biophysical Journal·Tsung-Yen LeeTsyr-Yan Yu
Jul 19, 2005·Journal of Molecular Biology·Hideo OkumuraTsutomu Kouyama
Dec 27, 2005·Biochimica Et Biophysica Acta·Janos K Lanyi
May 30, 2006·Biophysical Journal·Satoshi P TsunodaPeter Hegemann
Sep 14, 2000·Biochimica Et Biophysica Acta·S P Balashov
Feb 25, 2003·Annual Review of Biophysics and Biomolecular Structure·Jean-Philippe Cartailler, Hartmut Luecke
Jul 4, 2001·The Journal of Biological Chemistry·L S BrownJ L Spudich
Sep 29, 2001·The Journal of Biological Chemistry·B C LeeW D Hoff
Jun 19, 2004·Molecular Membrane Biology·Janos K Lanyi
Jul 25, 2000·Journal of Molecular Biology·H LueckeJ K Lanyi
Sep 26, 2021·Proceedings of the National Academy of Sciences of the United States of America·Denis MaagTomáš Kubař

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