Relationship of the 37,000- and 40,000-M(r) tryptic fragments of islet antigens in insulin-dependent diabetes to the protein tyrosine phosphatase-like molecule IA-2 (ICA512)

The Journal of Clinical Investigation
M A PaytonM R Christie

Abstract

Sera from patients with insulin-dependent diabetes immunoprecipitate 64,000-M(r) proteins, distinct from glutamate decarboxylase, that are cleaved to 37,000- and 40,000-M(r) fragments by trypsin. We investigated possible relationships between 37,000- or 40,000-M(r) fragments of antigen and the tyrosine phosphatase-like protein, IA-2 (ICA512). Antibodies from nondiabetic relatives bound differentially to 37,000- and 40,000-M(r) fragments indicating presence of distinct epitopes. Precursors of these fragments could be separated on immobilized lectins, suggesting different carbohydrate content. Levels of antibodies to 40,000-M(r) fragments were strongly associated with those to the intracellular domain of IA-2. Recombinant intracellular domain of IA-2 blocked binding of antibodies to 40,000-M(r) fragments expressed by insulinoma cells and partially blocked binding to 37,000-M(r) fragments. Furthermore, trypsinization of recombinant intracellular domain of IA-2 generated proteolytic fragments of identical M(r) to the 40,000-M(r) fragments of insulinoma antigen; 37,000-M(r) fragments were not generated. Thus, 40,000-M(r) fragments of islet autoantigen are derived from a protein similar or identical to the tyrosine phosphatase-like m...Continue Reading

References

Feb 1, 1992·Diabetes·D U RabinJ A Shapiro
Dec 1, 1991·The Journal of Clinical Endocrinology and Metabolism·L CastañoG S Eisenbarth
Sep 1, 1990·The Journal of Experimental Medicine·M R ChristieT L Delovitch
May 1, 1986·The Journal of Experimental Medicine·L LefrancoisI S Trowbridge
Jan 1, 1993·Annual Review of Biochemistry·K M Walton, J E Dixon

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Citations

Jan 5, 2002·Diabetes/metabolism Research and Reviews·D Mauricio, A de Leiva
Oct 1, 1996·Diabetologia·S GenoveseE Bonifacio
Oct 1, 1996·Diabetologia·M G DeSilvaM S Lan
Jul 25, 2012·Clinical and Experimental Medicine·Jan Novak, Lucie Novakova
Apr 14, 2005·Best Practice & Research. Clinical Endocrinology & Metabolism·Alberto Falorni, Annalisa Brozzetti
Nov 20, 2003·Journal of Autoimmunity·Massimo BearzattoEzio Bonifacio
Dec 29, 1999·Clinica Chimica Acta; International Journal of Clinical Chemistry·M MasudaJ Furmaniak
Dec 21, 2000·Journal of Immunological Methods·A ZavialovA Hinkkanen
Mar 4, 1998·Diabetes Research and Clinical Practice·M E FabregatR Gomis
Apr 1, 2004·Endocrinology and Metabolism Clinics of North America·Timothy I M Tree, Mark Peakman
Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·M S LanA L Notkins
Oct 26, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·G R VreugdenhilJ M Galama
Mar 5, 2003·Diabetes Technology & Therapeutics·William E WinterDesmond Schatz
Apr 5, 2012·Cold Spring Harbor Perspectives in Medicine·Bart O Roep, Mark Peakman
Apr 9, 1999·Acta Paediatrica. Supplement·K KidaK Nakamura
Nov 1, 1996·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·F Dotta, U Di Mario
Apr 3, 2002·The Journal of Clinical Investigation·Vissia VigliettaDavid A Hafler
Nov 24, 1999·The Journal of Clinical Investigation·M PeakmanR M Chicz
Aug 20, 2010·Clinical Trials : Journal of the Society for Clinical Trials·Polly J BingleyMichael W Steffes
May 12, 2007·The Review of Diabetic Studies : RDS·F Susan Wong, Li Wen
Jun 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Shin-ichi HarashimaAbner Louis Notkins
May 21, 2011·Expert Review of Clinical Immunology·Famara SanéDidier Hober
Sep 9, 2008·Expert Opinion on Biological Therapy·Hideki Garren
Nov 5, 2011·Current Opinion in Immunology·Bart O Roep, Mark Peakman
Sep 22, 2010·Research in Veterinary Science·Lucy J DavisonBrian Catchpole

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