Relationship of the optical coherence tomography signal to underlying retinal histology in the tree shrew (Tupaia belangeri)

Investigative Ophthalmology & Visual Science
Carla J AbbottMichael J Pianta

Abstract

To interpret the retinal origin of the optical coherence tomography (OCT) signal by objectively (i.e., minimal investigator bias) aligning in vivo OCT longitudinal reflectivity profiles (LRPs) with corresponding vertical histologic sections. The Zeiss StratusOCT system was used to obtain retinal B-scans in vivo in eyes from adult tree shrews. Subsequently, the retinas were fixed and embedded. Semithin vertical sections through the retina were obtained from the same locations as the LRPs. A statistical correlation procedure that accounted for axial tissue shrinkage determined the best relationship between features in the LRP and sublaminae boundaries in corresponding histology sections. For the optimal relationship, the three regions of high reflectivity in the inner OCT signal corresponded to (1) the nerve fiber and ganglion cell layers, (2) the inner plexiform layer and amacrine cell somas, and (3) the outer plexiform layer. The two regions of low reflectivity in the inner OCT signal corresponded to (1) the somas of Müller, bipolar, and horizontal cells in the inner nuclear layer and (2) the outer nuclear layer. The outer OCT signal had a region of high reflectivity that corresponded to the photoreceptor inner and outer segmen...Continue Reading

Citations

Aug 18, 2012·American Journal of Ophthalmology·Lejla VajzovicCynthia A Toth
May 29, 2012·Acta Neurologica Scandinavica·M K Heim, B E Gidal
Jul 26, 2015·Neuroimaging Clinics of North America·Jade S SchiffmanRosa A Tang
Apr 12, 2018·Current Eye Research·Ashutosh JnawaliLisa A Ostrin
Aug 2, 2019·Investigative Ophthalmology & Visual Science·Petr SoukupSimon Pot
Feb 13, 2020·Veterinary Ophthalmology·Ricardo Augusto PecoraAngélica de Mendonça Vaz Safatle
Sep 29, 2020·Translational Vision Science & Technology·Alison L HuckenpahlerRoss F Collery
Sep 17, 2013·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Stefan KoinzerJohann Roider
May 31, 2018·Investigative Ophthalmology & Visual Science·Benjamin S SajdakJoseph Carroll
Sep 10, 2019·Progress in Retinal and Eye Research·Daniela M VerraDavid Hicks

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.