PMID: 6982342Jan 1, 1982Paper

Relationship of transepithelial electrical potential to membrane potentials and conductance ratios in frog skin

The Journal of Membrane Biology
W Nagel, A Essig

Abstract

Previous studies in anuran epithelia have shown that, after clamping the transepithelial voltage in symmetrical sequences for 4-6 min there is near-constancy of the rate of active Na transport and the associated oxidative metabolism, with a near-linear potential dependence of both. Here we have investigated in frog skin the cellular electrophysiolgical events associated with voltage clamping (Vt = inside-outside potential). Increase and decrease of Vt produced converse effects, related directly to the magnitude of Vt. Hyperpolarization resulted in prompt decrease in inward transepithelial current It and increase in fractional outer membrane resistance fRo (as evaluated from small transient voltage perturbations) and in outer membrane potential Vo. Overshoot of Vo was followed by relaxation to a quasi-steady state in minutes. Changes in fRo were progressive, with half times of some 1-5 sec. Changes in transepithelial slope conductance gt were more variable, usually preventing precise evaluation of the outer and inner cell membrane conductances go and gi. Nevertheless, it was shown that go is related inversely to Vt and Vo. Presuming insensitivity of gi to Vt, the dependence of Go on Vo in the steady state much exceeds that predi...Continue Reading

References

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Citations

Apr 1, 1988·Pflügers Archiv : European journal of physiology·M L FlontaW Nagel
Nov 1, 1988·The Journal of Membrane Biology·W NagelA Essig
Sep 1, 1983·Biophysical Journal·J F García-DíazA Essig
Oct 1, 1985·Pflügers Archiv : European journal of physiology·W Van Driessche, W Zeiske
Sep 14, 1999·The American Journal of Physiology·I Lang, H Martens
Mar 1, 1989·The American Journal of Physiology·M M CivanT G O'Brien
Feb 1, 1986·The American Journal of Physiology·S Sariban-Sohraby, D J Benos

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