Relationship of trehalose accumulation with ethanol fermentation in industrial Saccharomyces cerevisiae yeast strains

Bioresource Technology
Pin-Mei WangXue-Chang Wu

Abstract

The protective effect and the mechanisms of trehalose accumulation in industrial Saccharomyces cerevisiae strains were investigated during ethanol fermentation. The engineered strains with more intercellular trehalose achieved significantly higher fermentation rates and ethanol yields than their wild strain ZS during very high gravity (VHG) fermentation, while their performances were not different during regular fermentation. The VHG fermentation performances of these strains were consistent with their growth capacity under osmotic stress and ethanol stress, the key stress factors during VHG fermentation. These results suggest that trehalose accumulation is more important for VHG fermentation of industrial yeast strains than regular one. The differences in membrane integrity and antioxidative capacity of these strains indicated the possible mechanisms of trehalose as a protectant under VHG condition. Therefore, trehalose metabolic engineering may be a useful strategy for improving the VHG fermentation performance of industrial yeast strains.

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Citations

Dec 6, 2014·Journal of Industrial Microbiology & Biotechnology·Ke ZhangDaoqiong Zheng
Jun 25, 2016·World Journal of Microbiology & Biotechnology·Yan-Zhen MeiWen-Wan Fang
Jun 9, 2020·World Journal of Microbiology & Biotechnology·David J Timson
Aug 18, 2020·Applied Biochemistry and Biotechnology·David J Timson, Joshua Eardley
Mar 15, 2018·AMB Express·Yingjie MiaoPeifang Weng
Jan 15, 2021·Scientific Reports·Lisa DenglerJennifer C Ewald
Mar 24, 2018·Bioresource Technology·Shizeng WangQipeng Yuan

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