Relationships between platelet counts, platelet volumes and reticulated platelets in patients with ITP: evidence for significant platelet count inaccuracies with conventional instrument methods

International Journal of Laboratory Hematology
M DiquattroI Menozzi

Abstract

The platelet count has a primary role in the diagnosis and treatment of idiopathic thrombocytopenic purpura (ITP). This study analysed the accuracy of ITP patient platelet counts determined by Abbott CD-Sapphire (impedance/optical) and Bayer Advia 120 (optical) analyses, compared with a reference immunoplatelet method. Instrument platelet estimates showed broad equivalence in the higher range of observed values, but significant discrepancies against the immunoplatelet count were seen when platelet counts were <10 x 10(9)/l. CD-Sapphire mean platelet volume (MPV) results revealed increased (>12 fl) platelet volumes in eight of eight ITP patients with counts of <20 x 10(9)/l compared with 6/6 and 5/13 patients with platelet counts of 20-50 and >50 x 10(9)/l. In contrast, Bayer Advia MPV values showed no relationship with the platelet count. Increased reticulated platelets were associated with an increasing CD-Sapphire MPV (R(2) = 0.61) and a decreasing platelet count. High (>40%) reticulated platelet values were seen in 9/9 patients with immunoplatelet counts of <20 x 10(9)/l compared with 0/19 patients with platelet counts above 20 x 10(9)/l. There may be a need for caution in the interpretation of platelet counts in ITP patient...Continue Reading

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Citations

Jul 25, 2014·Journal of Veterinary Internal Medicine·D SchwartzJ Kelley
Nov 3, 2009·International Journal of Laboratory Hematology·M AsanumaM Fujishima
Mar 15, 2012·Annals of Medicine·Avi LeaderMichael Lishner
Nov 30, 2011·Thrombosis Research·Johannes J M L Hoffmann
Feb 19, 2014·Orvosi hetilap·Olivér RoseroAttila Szijártó
Dec 20, 2012·Experimental and Therapeutic Medicine·Rui-Long XuShun-Hong Zhuang
Jun 27, 2013·Clinical Chemistry and Laboratory Medicine : CCLM·Lisa MeintkerStefan W Krause

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