PMID: 1212220Oct 1, 1975Paper

Relationships between the exchange of calcium and phosphate in isolated fat-cells

The Biochemical Journal
B R MartinJ Gliemann

Abstract

The uptake and the washout of 45Ca2+ and 32Pi is described in free fat-cells and whole epididymal fat-pads from fed rats. 2. In isolated fat-cells, the uptake of 45Ca2+ proceeds with an initial rapid phase of about 1 min duration, followed by a slower subsequent accumulation. In contrast with the rapid phase, the slow phase is inhibited by 2,4-dinitrophenol, warfarin, oligomycin and verapamil, shows saturation, and presumably represents transport across the plasma membrane. 3. The washout of 45Ca2+ from preloaded cells consists of a rapid (1 min) initial phase and a slow phase which is non-monoexponential, suggesting that the radioactive isotope is released from several cellular pools. 4. When Pi is omitted from the incubation medium, the slow phase of 45Ca uptake is almost abolished, and the washout of 45Ca from preloaded fat-cells is markedly accelerated. At elevated extracellular concentrations of Pi (2,4-6.2mM), the uptake of 45Ca is stimulated by 2-10-fold, and the release of the radioactive isotope from preloaded cells is inhibited. In whole epididymal fat-pads, variations in the extracellular concentration of Pi have no detectable effect on the uptake or the washout of 45Ca. 5. In isolated fat-cells, the accumulation of ...Continue Reading

Citations

Jul 5, 2006·Evolution; International Journal of Organic Evolution·Eric G DeChaine, Andrew P Martin
Feb 1, 1978·Biological Reviews of the Cambridge Philosophical Society·F L Bygrave
Apr 6, 1976·Pflügers Archiv : European journal of physiology·J SchnermannM Hermle
Sep 1, 1976·European Journal of Biochemistry·C SchudtD Pette
Aug 28, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Philippe G Cammisotto, Ludwik J Bukowiecki
Jan 31, 1983·Life Sciences·N Venkatesan, M B Davidson
Mar 21, 1983·Biochimica Et Biophysica Acta·R D Moore

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