Relative abundance of aldose reductase mRNA in rat lens undergoing development of osmotic cataracts.

Current Eye Research
I BekhorN J Unakar

Abstract

Aldose reductase (AR) messenger RNA concentration was determined in normal rat lens and in lens from rats fed a 50% galactose diet over a period of 20 days. The AR mRNA was detected by using a previously described AR cDNA clone. The relative concentration of the AR mRNA was estimated by cpm of 35S-UTP labeled antisense RNA hybridized to dot-blots prepared from cytosols isolated from single lens, decapsulated lens (cortex) and its respective capsule (epithelia). The results demonstrated that the concentration of the AR mRNA in the epithelium doubled over the 20 day period. Correspondingly, an increase in the concentration of the DNA was also observed, suggesting that the increase in epithelial cytosolic mRNA might be partially due to the increase in the number of epithelial cells occurring in lens undergoing cataractogenesis. The increase in AR mRNA in the epithelia was gradual, and it doubled by day 12 on galactose, while the increase in DNA was rapid and reached an optimum level by about day 4. By day 4 the cortex AR mRNA concentration increased, then rapidly decreased to insignificant levels by day 20. Changes in AR mRNA and in DNA following a high influx of galactose in the lens might suggest a heightened gene response to ch...Continue Reading

References

Feb 1, 1978·Experimental Eye Research·N J UnakarV N Reddy
Jul 1, 1989·Experimental Eye Research·H M ChengR G González
Apr 1, 1986·Metabolism: Clinical and Experimental·H M Cheng, R G González
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P S Thomas
May 1, 1983·Experimental Eye Research·N J Unakar, J Y Tsui
Jun 1, 1984·Diabetes·Y AkagiJ H Kinoshita
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·P C HentzenI Bekhor
Aug 1, 1957·American Journal of Ophthalmology·L VON SALLMANN
Jun 1, 1962·Experimental Eye Research·R VAN HAYNINGEN
Jun 1, 1962·Experimental Eye Research·J H KINOSHITAE DIKMAK

❮ Previous
Next ❯

Citations

Aug 1, 1993·The Journal of Clinical Investigation·D N HenryP D Killen
Oct 1, 1992·Biochemical Medicine and Metabolic Biology·A Bhatnagar, S K Srivastava
Aug 29, 1997·Journal of Toxicology and Environmental Health·P KhannaN H Ansari
Aug 31, 1992·Biochemical and Biophysical Research Communications·J M PetrashJ Bylander
Nov 1, 1994·Biochemical Pharmacology·R H GriffeyD L Vander Jagt
Jun 1, 1997·Glia·C Jacquin-Becker, G Labourdette
Jul 15, 2000·Arteriosclerosis, Thrombosis, and Vascular Biology·J RuefA Bhatnagar
Nov 14, 2002·American Journal of Veterinary Research·Marianne RichterBernhard Spiess

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.