Relative Apoptosis-inducing Potential of Homeopa-thic Condurango 6C and 30C in H460 Lung Cancer Cells In vitro: -Apoptosis-induction by homeopathic Condurango in H460 cells

Journal of Pharmacopuncture
Sourav SikdarAnisur Rahman Khuda-Bukhsh

Abstract

In homeopathy, it is claimed that more homeopathically-diluted potencies render more protective/curative effects against any disease condition. Potentized forms of Condurango are used successfully to treat digestive problems, as well as esophageal and stomach cancers. However, the comparative efficacies of Condurango 6C and 30C, one diluted below and one above Avogadro's limit (lacking original drug molecule), respectively, have not been critically analyzed for their cell-killing (apoptosis) efficacy against lung cancer cells in vitro, and signalling cascades have not been studied. Hence, the present study was undertaken. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenylte-trazolium bromide (MTT) assays were conducted on H460-non-small-cell lung cancer (NSCLC) cells by using a succussed ethyl alcohol vehicle (placebo) as a control. Studies on cellular morphology, cell cycle regulation, generation of reactive oxygen species (ROS), changes in mitochondrial membrane potential (MMP), and DNA-damage were made, and expressions of related signaling markers were studied. The observations were done in a "blinded" manner. Both Condurango 6C and 30C induced apoptosis via cell cycle arrest at subG0/G1 and altered expressions of certain apoptotic m...Continue Reading

References

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Citations

Aug 1, 2019·International Journal of Oncology·Jenifer Pendiuk GonçalvesCarolina Camargo De Oliveira
Dec 22, 2020·Homeopathy : the Journal of the Faculty of Homeopathy·Andreza Pereira Dos SantosLeoni Villano Bonamin

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Methods Mentioned

BETA
scanning electron microscopy
flowcytometry
light microscopy
ELISA
fluorescence

Software Mentioned

SPSS
image J

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