Relative contribution of homologous recombination and non-homologous end-joining to DNA double-strand break repair after oxidative stress in Saccharomyces cerevisiae

DNA Repair
Lucia LetavayováJela Brozmanová

Abstract

Oxidative damage to DNA seems to be an important factor in developing many human diseases including cancer. It involves base and sugar damage, base-free sites, DNA-protein cross-links and DNA single-strand (SSB) and double-strand (DSB) breaks. Oxidative DSB can be formed in various ways such as their direct induction by the drug or their generation either through attempted and aborted repair of primary DNA lesions or through DNA replication-dependent conversion of SSB. In general, two main pathways are responsible for repairing DSB, homologous recombination (HR) and non-homologous end-joining (NHEJ), with both of them being potential candidates for the repair of oxidative DSB. We have examined relative contribution of HR and NHEJ to cellular response after oxidative stress in Saccharomyces cerevisiae. Therefore, cell survival, mutagenesis and DSB induction and repair in the rad52, yku70 and rad52 yku70 mutants after hydrogen peroxide (H(2)O(2)), menadione (MD) or bleomycin (BLM) exposure were compared to those obtained for the corresponding wild type. We show that MD exposure does not lead to observable DSB induction in yeast, suggesting that the toxic effects of this agent are mediated by other types of DNA damage. Although H(...Continue Reading

References

Oct 1, 1977·Nucleic Acids Research·L F PovirkF Hutchinson
Nov 21, 1992·Annals of the New York Academy of Sciences·B N Ames, M K Shigenaga
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S C PopoffB Demple
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R J Steighner, L F Povirk
Jan 1, 1994·Annual Review of Biochemistry·B Demple, L Harrison
Apr 5, 1996·The Journal of Biological Chemistry·G J MagesE L Winnacker
Jan 1, 1995·Folia Microbiologica·G Gille, K Sigler
Nov 1, 1996·The Journal of Biological Chemistry·H FeldmannE L Winnacker
Dec 22, 1998·Chemical Research in Toxicology·K Charles, L F Povirk
Mar 5, 1999·Mutation Research·J CadetS Sauvaigo
Jun 5, 1999·Microbiology and Molecular Biology Reviews : MMBR·F Pâques, J E Haber
Aug 1, 2000·Mutation Research·A Memisoglu, L Samson
Jun 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·J O Blaisdell, S S Wallace
Aug 17, 2001·Mutation Research·A PastinkP H Lohman
May 15, 2002·Radiation Research·Betsy M SutherlandJacques Laval
Jun 28, 2002·Free Radical Biology & Medicine·Susan S Wallace
Sep 12, 2002·Biological Chemistry·Michael van den BoschAlbert Pastink
Nov 29, 2002·Microbiology and Molecular Biology Reviews : MMBR·Lorraine S Symington
Dec 17, 2002·Oncogene·Bruce Demple, Michael S DeMott
Jan 2, 2003·DNA Repair·Penny Jeggo, Peter O'Neill
Nov 26, 2003·Mutation Research·Svein Bjelland, Erling Seeberg
May 29, 2004·Mutation Research·Andrej Dudás, Miroslav Chovanec
Sep 24, 2004·The EMBO Journal·Christine Anne KochDaniel Durocher
Nov 13, 2004·FEMS Microbiology Reviews·Zuzana DudásováMiroslav Chovanec

❮ Previous
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Citations

Jul 4, 2012·Chemical Research in Toxicology·Dominika MánikováMiroslav Chovanec
Dec 31, 2009·Nucleic Acids Research·Sylvia SteiningerSimone Moertl
Jun 30, 2012·PloS One·Huafei ZouFerruccio Galbiati
Jun 19, 2013·PloS One·Gerson Shigeru KobayashiMaria Rita Passos-Bueno
Aug 16, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dominika MánikováMiroslav Chovanec
Jul 6, 2014·Microbiological Research·Gustavo SantoyoMa Del Carmen Orozco-Mosqueda
Apr 22, 2008·Mutation Research·Lucia LetavayováMiroslav Chovanec
Sep 29, 2007·Mutation Research·Lucia LetavayováMiroslav Chovanec
Apr 20, 2007·Biochemical and Biophysical Research Communications·Angela T Y TamJörg Heierhorst
Jul 21, 2009·The Biochemical Journal·Sara Maria Nancy Onnebo, Adolfo Saiardi
Nov 26, 2010·Trends in Genetics : TIG·Ashley FarlowChristian Schlötterer
Oct 10, 2007·Microbiological Research·Gustavo Santoyo, Jeffrey N Strathern
Jul 5, 2008·DNA Repair·Sylvia SteiningerSimone Moertl
Oct 12, 2018·International Journal of Radiation Biology·Liqiu MaShin Hatakeyama
Nov 14, 2018·PLoS Neglected Tropical Diseases·Danielle Gomes Passos SilvaCarlos Renato Machado
Dec 21, 2006·Molecular and Cellular Biochemistry·Joemerson Osório RosadoDiego Bonatto
Sep 18, 2020·Seminars in Cell & Developmental Biology·Isabel E Wassing, Fumiko Esashi

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