Relative contributions of recombination and mutation to the diversification of the opa gene repertoire of Neisseria gonorrhoeae.

Journal of Bacteriology
Nicole BilekBrian G Spratt

Abstract

To understand the rates and mechanisms of Neisseria gonorrhoeae opa gene variation, the 11 opa genes were amplified independently so that an opa allelic profile could be defined for any isolate from the sequences at each locus. The opa allelic profiles from 14 unrelated isolates were all different, with no opa alleles shared between isolates. Examination of very closely related isolates from sexual contacts and sexual networks showed that these typically shared most opa alleles, and the mechanisms by which recent changes occurred at individual opa loci could be determined. The great majority of changes were due to recombination among existing alleles that duplicated an opa allele present at another locus or resulted in a mosaic of existing opa alleles. Single nucleotide changes or insertion/deletion of a single codon also occurred, but few of these events were assigned to mutation, the majority being assigned to localized recombination. Introduction of novel opa genes from coinfecting strains was rare, and all but one were observed in the same sexual network. Changes at opa loci occurred at a greater rate than those at the porin locus, and the opa11 locus changed more rapidly than other opa loci, almost always differing even be...Continue Reading

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Citations

Oct 27, 2009·PLoS Computational Biology·Eva C BerglundSiv G E Andersson
May 30, 2015·Small GTPases·Cristián A QuinteroMaría T Damiani
Oct 10, 2014·Scientific Reports·Eleanor R WatkinsCaroline O Buckee
Jul 4, 2009·Microbiology·Peter T Beernink, Dan M Granoff
Jan 6, 2011·FEMS Microbiology Reviews·Manish SadaranganiScott D Gray-Owen
Sep 24, 2016·Future Microbiology·Eleanor R WatkinsSunetra Gupta
Jan 1, 2013·Biology·Miao-Chiu Hung, Myron Christodoulides
Jun 30, 2016·MBio·Xavier DidelotSimon R Harris
Jul 18, 2018·Antibiotics·Lilith K WhittlesXavier Didelot

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