Relative importance of newly synthesized and stored hormone to basal secretion by growth hormone and prolactin cells

Endocrinology
T T ChenL S Frawley

Abstract

It is generally accepted that under basal conditions there is preferential release of newly synthesized hormone by a number of endocrine cell type, including those that secrete GH or PRL. However, the cellular basis for this phenomenon along with the relative contribution of stored hormone to basal secretion has yet to be clearly established. In the present study, we employed reverse hemolytic plaque assays to monitor basal and stimulated release of GH and PRL from individual cells in which de novo protein synthesis had been blocked. Monodispersed pituitaries from adult male rats were cultured for 21 h in the absence or presence of maximally effective doses of puromycin (100 microM) or cycloheximide (36 microM) and were then subjected to separate plaque assays for GH or PRL. Treatment with puromycin reduced the percentage of GH or PRL secretors (plaque formers) by about half. Coincubation with stimulatory secretagogues did not increase the percentages of GH or PRL secretors in control cultures, but returned the proportion in puromycin-treated cells to normal, demonstrating that cells which failed to secrete basally could still release hormone from their stored pools when stimulated. Very similar results were obtained when these...Continue Reading

Citations

Apr 1, 1991·Molecular and Cellular Endocrinology·P MukherjeeW C Hymer
Apr 28, 2001·Molecular and Cellular Endocrinology·W K Yunker, J P Chang
May 31, 1993·Annals of the New York Academy of Sciences·F J van StrienE W Roubos
Dec 1, 1991·General and Comparative Endocrinology·D J Donoghue, C G Scanes
Nov 1, 1992·General and Comparative Endocrinology·J P CastañoF Gracia-Navarro
Aug 1, 1997·General and Comparative Endocrinology·W Lee, N L Wayne
May 6, 2004·Journal of Cell Science·Roslyn A BauerJoseph K Angleson

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