PMID: 9659612Jul 11, 1998Paper

Relative importance of surface wettability and charged functional groups on NIH 3T3 fibroblast attachment, spreading, and cytoskeletal organization

Journal of Biomedical Materials Research
K WebbP A Tresco

Abstract

Understanding the relationships between material surface properties, adsorbed proteins, and cellular responses is essential to designing optimal material surfaces for implantation and tissue engineering. In this study, we have prepared model surfaces with different functional groups to provide a range of surface wettability and charge. The cellular responses of attachment, spreading, and cytoskeletal organization have been studied following preadsorption of these surfaces with dilute serum, specific serum proteins, and individual components of the extracellular matrix. When preadsorbed with dilute serum, cell attachment, spreading, and cytoskeletal organization were significantly greater on hydrophilic surfaces relative to hydrophobic surfaces. Among the hydrophilic surfaces, differences in charge and wettability influenced cell attachment but not cell area, shape, or cytoskeletal organization. Moderately hydrophilic surfaces (20-40 degree water contact angle) promoted the highest levels of cell attachment. Preadsorption of the model surfaces with bovine serum albumin (BSA) resulted in a pattern of cell attachment very similar to that observed following preadsorption with dilute serum, suggesting an important role for BSA in re...Continue Reading

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Citations

Jul 25, 2000·Journal of Biomedical Materials Research·G AltankovM Ulbricht
Aug 22, 2000·Journal of Biomedical Materials Research·G HaipengZ Xiufang
Jun 28, 2001·Journal of Biomedical Materials Research·K WebbP A Tresco
Apr 12, 2002·Journal of Biomedical Materials Research·Robert A Latour, Christopher J Rini
Sep 11, 2002·Journal of Biomedical Materials Research·Kazuhiro Tanahashi, Antonios G Mikos
Sep 5, 2002·Journal of Biomedical Materials Research·Danielle C GilibertiKay C Dee
Dec 17, 2002·Journal of Biomedical Materials Research. Part a·Andrea L KoenigDavid W Grainger
Mar 22, 2013·Biomedical Microdevices·Katayoon SaadinIan M White
Mar 6, 2007·Journal of Materials Science. Materials in Medicine·V PesskováL Himmlová
Jun 5, 2007·Journal of Materials Science. Materials in Medicine·Helmut SchweiklGottfried Schmalz
Dec 25, 2007·Journal of Materials Science. Materials in Medicine·E A dos SantosK Anselme
Feb 3, 2011·Journal of Materials Science. Materials in Medicine·Marianne CrespinStephane Lucas
Aug 25, 2012·Journal of Materials Science. Materials in Medicine·D M GordinA Cimpean
Jan 5, 2002·Biomaterials·Sharon L SanbornKandice Kottke-Marchant
Jul 11, 2003·Biomaterials·Dai KatoMasashi Kunitake
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Nov 6, 2009·Journal of the American Chemical Society·Myung-Ryul LeeKristyn S Masters
Apr 2, 2010·Lab on a Chip·Min-Hsien WuGwo-Bin Lee
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May 25, 2012·Tissue Engineering. Part C, Methods·Jangho KimJong Hoon Chung
Jun 11, 2010·Journal of Biomaterials Science. Polymer Edition·Josna JosephP V Mohanan

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