Relative importance of three iron-regulated outer membrane proteins for in vivo growth of Vibrio cholerae.

Infection and Immunity
K T TashimaS B Calderwood

Abstract

Iron is an essential nutrient to support the growth of most bacterial species. However, iron is not easily available to microorganisms infecting mammalian hosts, because it is largely sequestered by iron-binding proteins, such as transferrin or lactoferrin, or complexed to heme. In response to environmental iron stress, Vibrio cholerae produces the siderophore vibriobactin as well as a number of iron-induced outer membrane proteins. Previous data on the role of iron acquisition systems for the intraintestinal growth of mucosal pathogens such as V. cholerae are conflicting. In this report, we isolated mutants of V. cholerae with TnphoA fusions in each of viuA, hutA, and irgA, as well as strains mutant in each pair of these genes and all three simultaneously, to analyze the role of these iron-induced outer membrane protein receptors for in vivo growth of V. cholerae. The fusion between hutA and TnphoA in a single copy on the chromosome allowed the study of in vitro regulation of hutA in response to iron, fur, and irgB; transcription of hutA was tightly iron regulated (70-fold) and dependent on a functional Fur but did not require IrgB. To investigate the effects of mutations in these iron-induced outer membrane proteins on in viv...Continue Reading

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Citations

Mar 16, 2007·Environmental Microbiology·Hiroshi AsakuraShizunobu Igimi
May 26, 2011·Folia Microbiologica·E Ruiz-BustosF Ascencio
Sep 6, 2006·Journal of Bacteriology·Elizabeth E WyckoffShelley M Payne
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Jan 12, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Elizabeth E WyckoffShelley M Payne
Oct 24, 2001·Journal of Bacteriology·F AeckersbergE G Ruby
Dec 20, 1999·Microbiology and Immunology·S YamamotoS Narimatsu
Jun 23, 2009·Journal of Bacteriology·Aleksandra E SikoraMaria Sandkvist
Nov 22, 2005·Infection and Immunity·Alexandra R MeyShelley M Payne
Jan 16, 2007·Infection and Immunity·Matthew S LawlorVirginia L Miller
Nov 28, 2001·Molecular Microbiology·A R Mey, S M Payne
Jun 7, 2005·Journal of Bacteriology·Brigid M DavisMatthew K Waldor
Feb 1, 2011·Journal of Bacteriology·Christa H ChatfieldNicholas P Cianciotto
Jul 28, 2011·Molecular Microbiology·Elizabeth E Wyckoff, Shelley M Payne
Aug 2, 2019·Nature·Fabian Rivera-Chávez, John J Mekalanos

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