Relative induction of heat shock protein in coronary endothelial cells and cardiomyocytes: implications for myocardial protection

The Journal of Thoracic and Cardiovascular Surgery
M AmraniM H Yacoub

Abstract

Induction of the 70 kd heat shock protein in the heart is known to exert a protective effect against postischemic mechanical and endothelial dysfunction. However, the exact site of induction and the mechanisms involved remain unknown. The aim of this study was to investigate the relative capacity of endothelial and myocardial cells to express the 70 kd heat shock protein in response to heat stress, as well as their significance. (1) Postischemic recovery of cardiac mechanical and endothelial function was studied in isolated rat hearts with and without endothelial denudation with saponin. (2) Semiquantitative determination of induction of 70 kd heat shock protein by Western immunoblotting was performed in the whole cardiac homogenate, in isolated cardiac myocytes, and in coronary endothelial cells. (3) Immunocytochemistry was used to visualize the distribution of induction of 70 kd heat shock protein in both cell types. Postischemic recovery (percent preischemic value +/- standard error of the mean) of cardiac output in hearts from heat-stressed animals was significantly improved (66.7 +/- 6.9 vs 44.5 +/- 4.5 in the control group, p < 0.01). In heat-stressed hearts treated with saponin no improvement in the recovery of cardiac o...Continue Reading

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Citations

Sep 29, 2011·Cell Stress & Chaperones·Jordan Thomas SilverEarl G Noble
Jun 6, 2000·Trends in Pharmacological Sciences·A Rubino, D M Yellon
Jul 10, 1999·The International Journal of Biochemistry & Cell Biology·C C GrayM H Yacoub
Apr 18, 2014·International Journal of Legal Medicine·E DoberentzB Madea
Mar 20, 2002·The Annals of Thoracic Surgery·Serban C StoicaStephen R Large
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Apr 10, 2007·Biochemical and Biophysical Research Communications·Jinah HwangSang Ick Park
Feb 28, 2004·Journal of Biomechanical Engineering·Sihong WangShanti J Aggarwal
Oct 27, 2005·Journal of Biomechanical Engineering·Marissa Nichole RylanderShanti J Aggarwal
May 18, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·David B ThorpEarl G Noble
May 6, 2017·Journal of Applied Physiology·Michael A FranciscoChristopher T Minson
Apr 28, 2006·Transplantation·Isabel QuirogaSusan V Fuggle
Mar 29, 2003·American Journal of Physiology. Heart and Circulatory Physiology·M Brennan HarrisRichard C Venema
Oct 3, 2001·Physiological Reviews·L H SnoeckxG J Van Der Vusse

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