Relative rates of non-pneumonic SARS coronavirus infection and SARS coronavirus pneumonia

Lancet
Patrick C Y WooKwok-yung Yuen

Abstract

Although the genome of severe acute respiratory syndrome coronavirus (SARS-CoV) has been sequenced and a possible animal reservoir identified, seroprevalence studies and mass screening for detection of subclinical and non-pneumonic infections are still lacking. We cloned and purified the nucleocapsid protein and spike polypeptide of SARS-CoV and examined their immunogenicity with serum from patients with SARS-CoV pneumonia. An ELISA based on recombinant nucleocapsid protein for IgG detection was tested with serum from 149 healthy blood donors who donated 3 years previously and with serum positive for antibodies against SARS-CoV (by indirect immunofluorescence assay) from 106 patients with SARS-CoV pneumonia. The seroprevalence of SARS-CoV was studied with the ELISA in healthy blood donors who donated during the SARS outbreak in Hong Kong, non-pneumonic hospital inpatients, and symptom-free health-care workers. All positive samples were confirmed by two separate western-blot assays (with recombinant nucleocapsid protein and recombinant spike polypeptide). Western-blot analysis showed that the nucleocapsid protein and spike polypeptide of SARS-CoV are highly immunogenic. The specificity of the IgG antibody test (ELISA with positi...Continue Reading

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Citations

Mar 30, 2005·Journal of Virological Methods·Masayuki SaijoShigeru Morikawa
Jul 28, 2006·Epidemiology and Infection·P A TuanUNKNOWN WHO SARS Investigation Team in Vietnam
Jun 16, 2004·Nature Reviews. Microbiology·B Brett FinlayRobert C Brunham
Mar 21, 2012·Proceedings of the National Academy of Sciences of the United States of America·Patrick C Y WooKwok-Yung Yuen
Nov 17, 2005·Journal of Neurovirology·Samson S Y Wong, K Y Yuen
Oct 16, 2007·Clinical Microbiology Reviews·Vincent C C ChengKwok Yung Yuen
Sep 9, 2011·Clinical and Vaccine Immunology : CVI·Anna J X ZhangKwok-Yung Yuen
Jun 8, 2006·Journal of Clinical Microbiology·Susanna K P LauKwok-yung Yuen
Sep 8, 2005·Journal of Clinical Microbiology·Susanna K P LauKwok-Yung Yuen
Oct 23, 2004·Emerging Infectious Diseases·I F N HungK Y Yuen
Jul 19, 2005·Emerging Infectious Diseases·Joke SpaargarenRoel A Coutinho
Jul 19, 2005·Emerging Infectious Diseases·Susanna K P LauKwok-Yung Yuen
Jul 19, 2005·Emerging Infectious Diseases·Annelies Wilder-SmithYee S Leo
Feb 21, 2006·Emerging Infectious Diseases·Ming WangJianguo Xu
Mar 25, 2014·Emerging Infectious Diseases·Patrick C Y WooKwok-Yung Yuen
Sep 20, 2005·Proceedings of the National Academy of Sciences of the United States of America·Susanna K P LauKwok-Yung Yuen
Feb 13, 2016·Nature Reviews. Drug Discovery·Alimuddin ZumlaKwok-Yung Yuen
Jul 26, 2013·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Jasper Fuk-Woo ChanPatrick Chiu-Yat Woo

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Methods Mentioned

BETA
ELISA
PCR

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