Relative sensitivity factors of inorganic cations in frozen-hydrated standards in secondary ion MS analysis

Analytical Chemistry
Cedric DérueC Ripoll

Abstract

We describe the measurement, at 100 K, of the SIMS relative sensitivity factors (RSFs) of the main physiological cations Na+, K+, Mg2+, and Ca2+ in frozen-hydrated (F-H) ionic solutions. Freezing was performed by either plunge freezing or high-pressure freezing. We also report the measurement of the RSFs in flax fibers, which are a model for ions in the plant cell wall, and in F-H ionic samples, which are a model for ions in the vacuole. RSFs were determined under bombardment with neutral oxygen (FAB) for both the fibers and the F-H samples. We show that referencing to ice-characteristic secondary ions is of little value in determining RSFs and that referencing to K is preferable. The RSFs of Na relative to K and of Ca relative to Mg in F-H samples are similar to their respective values in fiber samples, whereas the RSFs of both Ca and Mg relative to K are lower in fibers than in F-H samples. Our data show that the physical factors important for the determination of the RSFs are not the same in F-H samples and in homogeneous matrixes. Our data show that it is possible to perform a SIMS relative quantification of the cations in frozen-hydrated samples with an accuracy on the order of 15%. Referencing to K permits the quantificat...Continue Reading

References

Sep 14, 2001·Journal of Microscopy·D StuderP Eggli
Aug 31, 2002·Microscopy Research and Technique·Cedric DérueMichel Thellier

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Citations

Nov 5, 2011·Analytical and Bioanalytical Chemistry·Katie L MooreChris R M Grovenor
Nov 28, 2013·Analytical and Bioanalytical Chemistry·Shane R EllisRon M A Heeren
Feb 29, 2016·Micron : the International Research and Review Journal for Microscopy·Marcel Menezes Lyra da CunhaSergio Marco
Aug 12, 2016·Scientific Reports·Dan AokiKazuhiko Fukushima
Jan 3, 2007·Journal of Mass Spectrometry : JMS
Jun 22, 2010·The Plant Journal : for Cell and Molecular Biology·Katharine E SmartChris R M Grovenor
Jul 21, 2017·Scientific Reports·Wakaba OkumuraKazuhiko Fukushima
Apr 11, 2008·The Journal of Physical Chemistry. B·G LegentC Ripoll

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