PMID: 7537873Apr 11, 1995Paper

Relative stabilities of triple helices composed of combinations of DNA, RNA and 2'-O-methyl-RNA backbones: chimeric circular oligonucleotides as probes

Nucleic Acids Research
S Wang, E T Kool

Abstract

Described is a systematic study of the effects of varied backbone structure on the stabilities of pyr.pur.pyr triple helices. The effects were measured using six circular 34 base oligonucleotides containing DNA (D), RNA (R) and/or 2'-O-methyl-RNA (M) residues designed to bind a complementary single-stranded purine target strand by triple helix formation. Eighteen different backbone combinations were studied at pH 5.5 and 7.0 by optical melting experiments and the results compared with the stabilities of the corresponding Watson-Crick duplexes. When the target purine strand is DNA, all circles form pH-dependent triple helical complexes which are considerably stronger than the duplexes alone. When RNA is the target, five of the nine complexes studied are of the pH-dependent triplex type and the other four complexes are not significantly stronger than the corresponding duplexes. The results are useful in the design of the highest affinity ligands for single- and double-stranded DNAs and RNAs and also point out novel ways to engender DNA- or RNA-selective binding.

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Citations

Jun 1, 1997·Bioorganic & Medicinal Chemistry·S WangE T Kool
Dec 3, 2009·Bioconjugate Chemistry·Meghan A Campbell, Paul S Miller
Sep 20, 2002·Antisense & Nucleic Acid Drug Development·Dalila SekkaiJean-Jacques Toulmé
Jul 11, 2003·Nucleic Acids Research·Rachel A CassidyPaul S Miller
May 10, 2013·Nucleic Acids Research·Yuan ZhouGang Chen
Nov 1, 2012·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Li-Feng TanXiao-Nan Sun
Dec 1, 1996·Annals of Medicine·H Lönnberg, E Vuorio
Nov 24, 2004·Bioorganic & Medicinal Chemistry·Yoshihito UenoYukio Kitade
Dec 8, 2005·Bioorganic & Medicinal Chemistry Letters·Aya ShibataYukio Kitade
Aug 31, 2002·Analytical Biochemistry·Paula J BatesDonald M Miller
Aug 26, 2014·Wiley Interdisciplinary Reviews. RNA·Gitali DeviGang Chen
Sep 17, 2014·The Biochemical Journal·Weronika KotkowiakAnna Pasternak
Jun 11, 1999·Current Opinion in Structural Biology·D E Gilbert, J Feigon
May 11, 2018·Physical Chemistry Chemical Physics : PCCP·Belinda J BoehmDavid M Huang
Aug 18, 1998·Accounts of Chemical Research·Eric T Kool
Dec 18, 2003·Journal of the American Chemical Society·Anna AviñóModesto Orozco
Nov 1, 2001·Journal of the American Chemical Society·D P AryaI Charles

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