Relative susceptibilities of the glucosamine-glucuronic acid and N-acetylglucosamine-glucuronic acid linkages to heparin lyase III

Biochemistry
Wengang ChaiT Feizi

Abstract

Heparin lyases are valuable tools for generating oligosaccharide fragments and in sequence determination of heparan sulfate (HS). Heparin lyase III is known to cleave the linkages between N-acetylglucosamine (GlcNAc) or N-sulfated glucosamine (GlcNS) and glucuronic acid (GlcA) as the primary sites and the linkages between GlcNAc, GlcNAc(6S), or GlcNS and iduronic acid as secondary sites. N-Unsubstituted glucosamine (GlcN) occurs as a minor component in HS, and it has been associated with various bioactivities. Here we investigate the specificity of heparin lyase III toward the GlcN-GlcA linkage using a recombinant enzyme of high purity and as substrates the partially de-N-acetylated polysaccharide of Escherichia coli K5 strain and derived hexasaccharides. The specificity of lyase III toward the GlcN-GlcA linkage is deduced by sequencing of the oligosaccharide products using electrospray mass spectrometry with collision-induced dissociation and MS/MS scanning. The results demonstrate that under controlled conditions for partial digestion, lyase III does not act at the GlcN-GlcA linkage, whereas GlcNAc-GlcA is cleaved. Even under forced conditions for exhaustive digestion, the GlcN-GlcA linkage is only partly cleaved. It is this ...Continue Reading

References

Nov 1, 1992·The Journal of Cell Biology·G DavidH Van den Berghe
Jul 15, 1985·The Biochemical Journal·Z M MerchantR J Linhardt
Dec 29, 1995·The Journal of Biological Chemistry·J van den BornU Lindahl
Mar 30, 2001·The Journal of Biological Chemistry·C LeteuxT Feizi
Oct 2, 2003·The Journal of Biological Chemistry·Balagurunathan KuberanRobert D Rosenberg

❮ Previous
Next ❯

Citations

Mar 1, 2006·Journal of the American Society for Mass Spectrometry·Zhenqing ZhangWengang Chai
Feb 27, 2009·The Journal of Biological Chemistry·Xiaofeng Shi, Joseph Zaia
Dec 14, 2005·Rapid Communications in Mass Spectrometry : RCM·Toshikazu MinamisawaJun Hirabayashi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biosynthetic Transformations

Biosyntheic transformtions are multi-step, enzyme-catalyzed processes where substrates are converted into more complex products in living organisms. Simple compounds are modified, converted into other compounds, or joined together to form macromolecules. Discover the latest research on biosynthetic transformations here.