Relative timing of redistribution of cytochrome c and Smac/DIABLO from mitochondria during apoptosis assessed by double immunocytochemistry on mammalian cells

Experimental Cell Research
Maria L R LimPhillip Nagley

Abstract

Redistribution of cytochrome c and Smac/DIABLO from mitochondria occurs during apoptosis, although the relative timing of their release is not well characterized. Double immunocytochemistry was utilized here to study quantitatively the patterns of release of cytochrome c and Smac/DIABLO from mitochondria in single cells. Human osteosarcoma cells and murine embryonic cortical neurons were analyzed during apoptosis induced by staurosporine. In osteosarcoma cells treated with staurosporine for 24 h, a substantial proportion of cells (36%) released cytochrome c from the mitochondria before Smac/DIABLO. In contrast, these proteins were released mostly concordantly in neurons; only a minority of cells (< or = 15%) released cytochrome c without Smac/DIABLO (or vice versa) from mitochondria. Patterns of release in either cell type were unaltered by addition of the caspase inhibitor, zVAD-fmk. The double immunocytochemistry procedure facilitated clear definition of the temporal release of cytochrome c and Smac/DIABLO from mitochondria in intact apoptotic cells, enabling us to demonstrate for the first time that their mutual redistribution during apoptosis varies between different cell types.

References

Aug 10, 2000·Trends in Cell Biology·S Desagher, J C Martinou
May 18, 2001·The Journal of Biological Chemistry·D ChauhanK C Anderson
Dec 14, 2001·The Journal of Biological Chemistry·Muniswamy MadeshGyörgy Hajnóczky
Jan 31, 2002·Proceedings of the National Academy of Sciences of the United States of America·Martin OttSten Orrenius
Jun 13, 2002·Clinical and Experimental Pharmacology & Physiology·John Ludbrook
Jul 18, 2002·The Journal of Biological Chemistry·Marion MacFarlaneGerald M Cohen
Oct 5, 2002·The Journal of Biological Chemistry·Stacy L SpringsGeorge L McLendon
Sep 17, 2003·The Journal of Cell Biology·Markus RehmJochen H M Prehn
Nov 19, 2003·The Journal of Cell Biology·Patrick Ryan PottsMohanish Deshmukh
Apr 13, 2004·Oncogene·Xavier SaelensPeter Vandenabeele
Nov 26, 2004·Cell Biology International·Magdalena GorkaTomasz Motyl
Apr 22, 2005·Apoptosis : an International Journal on Programmed Cell Death·L L ZhouD C Chang

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Citations

Sep 19, 2006·PLoS Computational Biology·Stefan LegewieHanspeter Herzel
Jul 8, 2009·Cellular and Molecular Life Sciences : CMLS·Gavin C HigginsPhillip Nagley
Mar 26, 2011·Cellular and Molecular Life Sciences : CMLS·Gavin C HigginsPhillip Nagley
Aug 17, 2010·Mitochondrion·Lina Ghibelli, Marc Diederich
May 7, 2009·Journal of Neurochemistry·Shanti DiwakarlaPhilip M Beart
Sep 16, 2009·Biochimica Et Biophysica Acta·Phillip NagleyPhilip M Beart
Jun 5, 2007·Oncogene·G Ceballos-CancinoJ Melendez-Zajgla
Apr 22, 2008·IUBMB Life·Danielle J SmithPhillip Nagley
Sep 12, 2013·Biotechnology and Bioengineering·Magesh ThiyagarajanXavier F Gonzales
Jul 22, 2014·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Carolina CanapèPernille Rose Jensen

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