PMID: 8385350Apr 1, 1993Paper

Relaxase (TraI) of IncP alpha plasmid RP4 catalyzes a site-specific cleaving-joining reaction of single-stranded DNA

Proceedings of the National Academy of Sciences of the United States of America
W PansegrauE Lanka

Abstract

Conjugative DNA transfer of the self-transmissible broad-host-range plasmid RP4 is initiated by strand- and site-specific cleavage at the nick site (nic) of the transfer origin (oriT). Cleavage results in covalent attachment of the plasmid-encoded relaxase (TraI) to the 5'-terminal 2'-deoxycytidine residue at nic. We demonstrate that Tyr22 is the center of the catalytic site of TraI, mediating cleavage via formation of a phosphodiester between the DNA 5' phosphoryl and the aromatic hydroxyl group. The specificity of cleavage seen with form I oriT DNA was verified with short oligodeoxy-ribonucleotides embracing the nick region. The reaction requires TraI and Mg2+ but is independent of the relaxosome component TraJ. Cleavage produces one oligonucleotide fragment with a free 3' hydroxyl, the other part forms a covalent TraI-oligonucleotide adduct. Like nicking of form I oriT DNA, TraI-catalyzed oligonucleotide cleavage reaches an equilibrium when about 30% of the input TraI exists as a covalent protein-DNA complex. In the presence of two differently sized oligonucleotides, defined hybrid oligonucleotides are produced, demonstrating that TraI catalyzes recombination of two single strands at nic. This finding shows that TraI possess...Continue Reading

References

Feb 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·V L WatersD G Guiney
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·W PansegrauE Lanka
May 1, 1990·Electrophoresis·R F JohnstonD L Barker
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J P FürsteE Lanka
Jan 1, 1968·Cold Spring Harbor Symposia on Quantitative Biology·M Ohki, J Tomizawa

❮ Previous
Next ❯

Citations

Oct 12, 2010·Biomolecular NMR Assignments·Nathan T WrightJoel F Schildbach
Feb 1, 1996·Trends in Microbiology·S C WinansP J Christie
Aug 6, 2000·The Plant Journal : for Cell and Molecular Biology·J ZupanP Zambryski
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·W PansegrauE Lanka
Oct 25, 1995·Nucleic Acids Research·A M VogelA Das
Nov 27, 2010·Nucleic Acids Research·Lubomír DostálJoel F Schildbach
May 8, 2010·Nucleic Acids Research·Rebekah Potts NashMatthew R Redinbo
Aug 19, 2006·Journal of Bacteriology·Daniel Pérez-MendozaJuan Sanjuán
Sep 22, 2009·Journal of Bacteriology·Sanja MihajlovicEllen L Zechner
Apr 30, 2013·Journal of Bacteriology·Cris Fernández-LópezD Roeland Boer
Feb 24, 2001·Journal of Bacteriology·W KarlE L Zechner
May 20, 2004·Journal of Bacteriology·Jamie A CarylChristopher D Thomas
Jan 5, 2005·Journal of Bacteriology·Steven W Matson, Heather Ragonese
May 7, 2009·Future Microbiology·Gayatri Vedantam
Sep 25, 2003·Proceedings of the National Academy of Sciences of the United States of America·Matthew J Harley, Joel F Schildbach
Dec 24, 1996·Proceedings of the National Academy of Sciences of the United States of America·P Bundock, P J Hooykaas
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·E L ZechnerG Högenauer
Mar 1, 1995·Molecular Microbiology·Y LuoR C Deonier
Apr 28, 2009·FEMS Microbiology Reviews·María Pilar Garcillán-BarciaFernando de la Cruz
Feb 14, 2006·FEMS Microbiology Reviews·Susan MillsR Paul Ross
Feb 21, 2004·FEMS Microbiology Reviews·M Victoria FranciaFernando de la Cruz
Apr 6, 2012·Mobile Genetic Elements·Mai Nguyen, Gayatri Vedantam
Dec 18, 1995·FEBS Letters·J LaufsB Gronenborn
Apr 23, 2016·Molecular Microbiology·Jolanta GodziszewskaGrazyna Jagura-Burdzy
Feb 5, 2008·Plasmid·Kurt JerkeAllan Konopka
Nov 13, 2015·Molecular Microbiology·Jessica A WisniewskiJulian I Rood
Mar 14, 2017·Plasmid·Jessica A Wisniewski, Julian I Rood

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Epigenetics Insights from Twin Studies

Find the latest research on epigenetics and twin studies here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.