Relaxin influences ileal muscular activity through a dual signaling pathway in mice

World Journal of Gastroenterology : WJG
Eglantina IdrizajMaria C Baccari

Abstract

To investigate the signaling pathways involved in the relaxin (RLX) effects on ileal preparations from mice through mechanical and electrophysiological experiments. For mechanical experiments, ileal preparations from female mice were mounted in organ baths containing Krebs-Henseleit solution. The mechanical activity was recorded via force-displacement transducers, which were coupled to a polygraph for continuous recording of isometric tension. Electrophysiological measurements were performed in current- and voltage-clamp conditions by a microelectrode inserted in a single smooth muscle cell (SMC) of the ileal longitudinal layer. Both the membrane passive properties and inward voltage-dependent L-type Ca2+ currents were recorded using suitable solutions and voltage stimulation protocols. Mechanical experiments showed that RLX induced a decay of the basal tension and a reduction in amplitude of the spontaneous contractions. The effects of RLX were partially reduced by 1H-[1,2,4]oxadiazolo[4,3-a]-quinoxalin-1-one (ODQ) or 9-cyclopentyladenine mesylate (9CPA), inhibitors of guanylate cyclase (GC) and adenylate cyclase (AC), respectively, and were abolished in the concomitant presence of both drugs. Electrophysiological experiments ...Continue Reading

References

Jan 1, 1981·Endocrine Research Communications·B M Sanborn, O D Sherwood
Jan 1, 1982·Endocrinology·R C Mercado-SimmenF C Greenwood
Jan 1, 1997·General Pharmacology·D Bani
Feb 10, 1999·The American Journal of Physiology·A IbrahimiR A Johnson
Apr 1, 1999·Annual Review of Physiology·T B BoltonD V Gordienko
Mar 1, 2002·Biology of Reproduction·Daniele BaniTatiana Bani Sacchi
Jul 18, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jacqueline NovakKirk P Conrad
Feb 18, 2003·Fundamental & Clinical Pharmacology·Patrick Vallance
Apr 16, 2003·Annals of Medicine·Jens SchlossmannFranz Hofmann
Oct 3, 2003·Biology of Reproduction·Maria Caterina BaccariDaniele Bani
Feb 18, 2004·Current Protein & Peptide Science·M C Baccari, F Calamai
Apr 15, 2004·Endocrine Reviews·O David Sherwood
Jun 25, 2004·Biology of Reproduction·Maria Caterina BaccariFranco Calamai
Jul 24, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Kirk P Conrad, Jacqueline Novak
Nov 9, 2004·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Tatsuya UenoMichael G Sarr
Feb 20, 2007·Circulation Research·Kenji Omori, Jun Kotera
May 25, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·M C BaccariD Bani
Dec 17, 2008·Current Protein & Peptide Science·M C Baccari, D Bani
Sep 14, 2011·The Journal of Physiology·Roberta SqueccoMaria Caterina Baccari
Sep 29, 2011·Cellular and Molecular Life Sciences : CMLS·Manuel MorgadoIgnacio Verde
Apr 28, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Xue-Dao He, Raj K Goyal
Aug 31, 2012·American Journal of Physiology. Endocrinology and Metabolism·M C BaccariM G Vannucchi
Jan 11, 2013·Physiological Reviews·R A D BathgateR J Summers
Aug 31, 2013·American Journal of Physiology. Gastrointestinal and Liver Physiology·Roberta SqueccoMaria Caterina Baccari
Oct 26, 2016·Current Medicinal Chemistry·Dieter GronebergAndreas Friebe
Sep 4, 2016·British Journal of Pharmacology·C H LeoL J Parry
Nov 12, 2016·Frontiers in Pharmacology·Maggie LamJane E Bourke
Apr 28, 2017·British Journal of Pharmacology·R J Summers
May 5, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Kelly YamasatoGillian D Bryant-Greenwood
Jun 14, 2017·Current Protein & Peptide Science·Rachele GarellaMaria Caterina Baccari

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Citations

Jan 22, 2019·Current Protein & Peptide Science·Eglantina IdrizajMaria Caterina Baccari
Jan 25, 2020·British Journal of Pharmacology·Damian D GuerraK Joseph Hurt
Aug 27, 2019·Frontiers in Physiology·Eglantina IdrizajRoberta Squecco
Sep 27, 2018·World Journal of Gastroenterology : WJG·Eglantina IdrizajMaria Caterina Baccari
Dec 23, 2020·International Journal of Molecular Sciences·Eglantina IdrizajMaria Caterina Baccari

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