Relaxin stimulation of porcine granulosa cell deoxyribonucleic acid synthesis in vitro: interactions with insulin and insulin-like growth factor I

Endocrinology
Q Zhang, C A Bagnell

Abstract

Relaxin (RLX) is a product of the theca interna layer of the developing pig follicle. However, its physiological role within the ovary is unknown. The purpose of this study was to determine the effects of RLX alone or in conjunction with insulin and insulin-like growth factor-I (IGF-I) on porcine granulosa cell (GC) DNA synthesis and proliferation in vitro. The results showed RLX (1-1000 ng/ml), insulin (1-1000 ng/ml), and IGF-I (1-30 ng/ml) alone stimulated [3H]thymidine incorporation into DNA by GC from small, medium, and large porcine follicles (P < 0.05). The effect of RLX in combination with insulin or with IGF-I on DNA synthesis by GC from small and medium follicles was greater than the effect of either single factor (P < 0.05). In the large follicle, RLX increased GC DNA synthesis synergistically with insulin and IGF-I. FSH (100 ng/ml) or estradiol (10(-7) M) had no effect on basal or RLX-induced [3H]thymidine incorporation into GC DNA at any stage of follicular development. After 6 days incubation in vitro, RLX dose-dependently increased proliferation of GC from small follicles (P < 0.05). The increased cell number in response to RLX correlates with RLX-induced [3H]thymidine incorporation into DNA by these GC. Hydroxyur...Continue Reading

Citations

Dec 10, 2009·The Journal of Veterinary Medical Science·Huen Suk KimWoo Suk Hwang
Aug 1, 1998·Biology of Reproduction·G Min, O D Sherwood
Nov 29, 2005·Reviews in Endocrine & Metabolic Disorders·Jae-Il ParkSheau Yu Teddy Hsu
Jun 16, 2001·Endocrine Reviews·S F PalterE Y Adashi
Apr 15, 2004·Endocrine Reviews·O David Sherwood

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