Relaxin treatment of solid tumors: effects on electric field-mediated gene delivery.

Molecular Cancer Therapeutics
Joshua W HenshawFan Yuan

Abstract

Pulsed electric fields have been shown to enhance interstitial transport of plasmid DNA (pDNA) in solid tumors in vivo. However, the extent of enhancement is still limited partly due to the collagen component in extracellular matrix. To this end, effects of collagen remodeling on interstitial electrophoresis were investigated by pretreatment of tumor-bearing mice with a recombinant human relaxin (rh-Rlx). In the study, two tumor lines (4T1 and B16.F10) were examined and implanted s.c. to establish two murine models: dorsal skin-fold chamber (DSC) and hind leg. Effects of rh-Rlx on pDNA electrophoresis were measured either directly in the DSC model or indirectly in the hind leg model via reporter gene expression. It was observed that rh-Rlx treatment reduced collagen levels in the hind leg tumors but not in the DSC tumors. The observation correlated with the results from electromobility experiments, where rh-Rlx treatment enhanced transgene expression in 4T1 hind leg tumors but did not increase the electromobility of pDNA in the DSC tumors. In addition, it was observed that pDNA binding to collagen could block its diffusion in collagen gel in vitro. These observations showed that effects of rh-Rlx on the collagen content depende...Continue Reading

References

Jan 1, 1985·Annals of the New York Academy of Sciences·L A Liotta, C N Rao
May 1, 1984·Acta Endocrinologica·I WiqvistN Wiqvist
Aug 1, 1995·Biology of Reproduction·G BaniT Bani Sacchi
Mar 8, 1995·Biochemical and Biophysical Research Communications·M P RolsJ Teissie
Jan 1, 1997·General Pharmacology·D Bani
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·L M MirD Scherman
May 20, 2000·Gene Therapy·J M WellsS W Hui
Nov 22, 2000·American Journal of Physiology. Heart and Circulatory Physiology·X Y ZhangF Yuan
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·A PluenR K Jain
Jul 17, 2001·American Journal of Physiology. Heart and Circulatory Physiology·S McGuire, F Yuan
May 25, 2002·Molecular Therapy : the Journal of the American Society of Gene Therapy·M Lee LucasRichard Heller
Jul 12, 2002·Current Gene Therapy·S Li, Z Ma
Aug 31, 2002·Biophysical Journal·Saroja RamanujanRakesh K Jain
Apr 1, 1961·The Journal of Physiology·A G OGSTON, T F SHERMAN
Feb 26, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Hagit Cohen-SacksGershon Golomb
Aug 13, 2005·Advances in Genetics·Lluis M MirJulie Gehl
Nov 22, 2005·Expert Opinion on Drug Delivery·Loree C HellerRichard Heller
Mar 23, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jaehwa ChoiMichael F Flessner
Jun 23, 2006·Annals of Biomedical Engineering·Sarah McGuireFan Yuan
Sep 16, 2006·Human Gene Therapy·Loree C Heller, Richard Heller
Oct 19, 2006·Journal of the National Cancer Institute·Joo-Hang KimChae-Ok Yun
Oct 31, 2006·BioTechniques·Chilman Bae, Peter J Butler
Nov 1, 2006·Current Pharmaceutical Design·M CemazarJ Teissié
Jul 13, 2007·Journal of Pharmaceutical Sciences·Joshua W Henshaw, Fan Yuan
Aug 31, 2007·Bioelectrochemistry·Joshua W HenshawFan Yuan

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Citations

Jan 18, 2012·Nanomedicine : Nanotechnology, Biology, and Medicine·Sarah McGuire, Fan Yuan
Aug 29, 2013·Journal of Biomedical Materials Research. Part a·Lin ZhangBochu Wang
Oct 20, 2018·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Liangli WangFan Yuan
Jun 12, 2018·Molecular Pharmaceutics·Lisa D Cervia, Fan Yuan

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