Relaxing effect of interleukin-1 on rat cultured Ito cells

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
M SakamotoK Tanikawa

Abstract

Interleukin-1beta (IL-1beta) is closely involved in liver disorders. IL-1beta produces nitric oxide (NO) in vascular smooth muscle cells and relaxes vascular smooth muscle via cyclic guanosine 3',5'-monophosphate (cGMP). In this study, we evaluated the relaxing effect of IL-1beta on cultured Ito cells. Ito cells were isolated from the livers of male Wistar rats and cultured for 24 hours. Immunolocalization of inducible nitric oxide synthase (iNOS) and cGMP and intensity of fluorescence of cGMP were examined using a confocal laser microscope. Ito cells were treated with 0, 200, and 1,000 pmol/L IL-1beta, and the intracellular cGMP concentration was measured after 12 hours. Moreover, Ito cells treated with 200 and 1,000 pmol/L IL-1beta and not treated with IL-1beta were observed over 12 hours, and the area of the same Ito cell was compared before and after the addition of IL-1beta. Next, effects of N(G)-monomethyl-L-arginine (L-NMMA) and S-nitroso-N-acetyl-DL-penicillamine (SNAP) on Ito cell relaxation by IL-1beta treatment were examined. In Ito cells, immunofluorescence of iNOS was observed, and fluorescent intensity of cGMP increased after addition of IL-1beta. Intracellular cGMP concentration increased dose-dependently after a...Continue Reading

Citations

Jun 9, 2001·Antioxidants & Redox Signaling·J D LaskinD L Laskin
Nov 6, 2008·Clinics in Liver Disease·Russell K Soon, Hal F Yee
Sep 24, 2004·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Takato UenoJean Rosenbaum
Mar 1, 2005·European Journal of Clinical Investigation·M SakamotoM Sata
May 20, 2008·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Hendrik ReynaertAlbert Geerts
Aug 14, 1999·Journal of Biological Rhythms·I V ZhdanovaJ A Taylor
Aug 14, 1999·The Journal of Biological Chemistry·J TaoS Lotersztajn
Aug 4, 1998·The American Journal of Physiology·C HellerbrandD A Brenner

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