Abstract
NF-kappa B was originally identified as a B cell-specific nuclear factor binding to the intronic kappa-light-chain enhancer element. It is found constitutively in the nucleus of mature B and plasma cells. In other cell types including pre-B cells, NF-kappa B is sequestered in the cytoplasm but can be induced by a variety of stimuli. In contrast to essentially all other mature B cells and plasma cell lines, the S107 plasmacytoma cell line lacks both constitutive and inducible kappa B-binding activity. A molecular characterization of the defect in these S107 cells suggests that the primary defect lies in the signal transduction pathway leading to NF-kappa B induction. Ectopic expression of RelB after stable transfection of these cells restores constitutive nuclear kappa B-binding activity. Moreover, kappa B-dependent transcription is also restored. Finally we demonstrate, that in contrast to parental S107 cells, the stable RelB transfectants have also regained the ability to specifically demethylate a transfected immunoglobulin kappa-locus. These data suggest that RelB is critically involved in both B cell-specific transcription and demethylation directed by the intronic kappa-enhancer element.
References
May 1, 1992·The Journal of Experimental Medicine·R SchreckP A Baeuerle
Jan 1, 1992·Free Radical Research Communications·R SchreckP A Baeuerle
Apr 20, 1990·Cell·U Zabel, P A Baeuerle
Dec 1, 1988·Baillière's Clinical Rheumatology·J P Pandey
Jan 16, 1987·Cell·M L Atchison, R P Perry
Jun 19, 1987·Science·M LenardoD Baltimore
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J W PierceD Baltimore
Apr 22, 1988·Cell·P A Baeuerle, D Baltimore
Oct 28, 1988·Science·P A Baeuerle, D Baltimore
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·Y BergmanD Baltimore
Oct 21, 1993·Nature·T LernbecherT Wirth
Jan 27, 1995·Cell·F WeihR Bravo
Feb 9, 1995·Nature·L BurklyD Lo
Mar 10, 1995·Science·K BrownU Siebenlist
Jan 1, 1994·Annual Review of Immunology·P A Baeuerle, T Henkel
Mar 11, 1994·Cell·M LichtensteinY Bergman
Aug 1, 1996·Nature Genetics·A KirillovY Bergman
Jan 1, 1996·Annual Review of Immunology·A S Baldwin
Oct 1, 1996·The Journal of Experimental Medicine·C M SnapperF Weih
Dec 1, 1996·Immunity·D C SchererE M Oltz
May 7, 1998·The International Journal of Biochemistry & Cell Biology·C XiaP Woo
Citations
Dec 26, 2001·European Journal of Immunology·Sandrine GuerinPhilippe Naquet
Jan 28, 2004·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Moshe Szyf
Oct 31, 2003·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Matthew Inlay, Yang Xu
Oct 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·B J O'SullivanR Thomas
Mar 14, 2009·Differentiation; Research in Biological Diversity·Christof Niehrs
Jun 13, 1998·The Journal of Biological Chemistry·B BaumannT Wirth
Jun 14, 2005·Biochemistry. Biokhimii︠a︡·M Szyf
Oct 19, 1999·Molecular and Cellular Biology·Y XiaL Feng
Apr 5, 2018·Epigenomics·Lisa BoureauMoshe Szyf