Release and extracellular metabolism of ATP by ecto-nucleotidase eNTPDase 1-3 in hypothalamic and pituitary cells.

Purinergic Signalling
Mu-Lan HeStanko S Stojilkovic

Abstract

Hypothalamic and pituitary cells express G protein-coupled adenosine and P2Y receptors and cation-conducting P2X receptor-channels, suggesting that extracellular ATP and other nucleotides may function as autocrine and/or paracrine signaling factors in these cells. Consistent with this hypothesis, we show that cultured normal and immortalized pituitary and hypothalamic cells release ATP under resting conditions. RT-PCR analysis also revealed the presence of transcripts for ecto-nucleotidase eNTPDase 1-3 in these cells. These enzymes were functional as documented by degradation of endogenously released and exogenously added ATP. Blocking the activity of eNTPDases by ARL67156 led to an increase in ATP release in perifused pituitary cells and inhibition of degradation of extracellularly added ATP. Furthermore, the addition of apyrase, a soluble ecto-nucleotidase, and the expression of recombinant mouse eNTPDase-2, enhanced degradation of both endogenously released and exogenously added ATP. The released ATP by resting hypothalamic cells was sufficient to activate and desensitize high-affinity recombinant P2X receptors, whereas facilitation of ATP metabolism by the addition of apyrase protected their desensitization. These results i...Continue Reading

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Sep 13, 2003·The Journal of Biological Chemistry·Mu-Lan HeStanko S Stojilkovic

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Citations

May 14, 2011·Purinergic Signalling·Qiang YuGeoffrey Burnstock
Nov 23, 2013·Purinergic Signalling·Geoffrey Burnstock
Aug 15, 2015·Molecular Reproduction and Development·Angélica S Martínez-Ramírez, Francisco G Vázquez-Cuevas
Aug 26, 2016·Molecular Neurobiology·Luana de Almeida-PereiraLucianne Fragel-Madeira
Oct 6, 2009·Trends in Endocrinology and Metabolism : TEM·Stanko S Stojilkovic
Apr 9, 2017·Biochimica Et Biophysica Acta. Biomembranes·Shuo LiStanko S Stojilkovic
Jul 3, 2007·British Journal of Pharmacology·S A LévesqueJ Sévigny
Aug 3, 2016·Molecular Endocrinology·Krystal Allen-WorthingtonMark S Roberson
Jul 24, 2010·Endocrine Reviews·Stanko S StojilkovicRichard Bertram
Apr 13, 2007·Physiological Reviews·Geoffrey Burnstock
Apr 22, 2006·Molecular Pharmacology·Elena SokolovaRashid Giniatullin
Apr 7, 2011·Endocrinology·Shuo LiStanko S Stojilkovic
Dec 17, 2009·American Journal of Physiology. Endocrinology and Metabolism·Hana ZemkovaStanko S Stojilkovic
Sep 28, 2013·Wiley Interdisciplinary Reviews. Membrane Transport and Signaling·Stanko S Stojilkovic, Hana Zemkova
Jun 27, 2017·Frontiers in Endocrinology·Stanko S StojilkovicHana Zemkova
Jan 24, 2012·Biochimica Et Biophysica Acta·Alberto Baroja-MazoPablo Pelegrín
Jul 8, 2017·Molecular and Cellular Endocrinology·Hana Zemková, Stanko S Stojilkovic

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Methods Mentioned

BETA
PCR
electrophoresis
transfection
Fluorescence Microscopy

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