Release and utilization of N-acetyl-D-glucosamine from human milk oligosaccharides by Bifidobacterium longum subsp. infantis.

Anaerobe
Daniel GarridoDavid A Mills

Abstract

Human milk contains high amounts of complex oligosaccharides, which can be utilized especially by Bifidobacterium species in the infant gut as a carbon and energy source. N-acetyl-D-glucosamine is a building block of these oligosaccharides, and molecular details on the release and utilization of this monosaccharide are not fully understood. In this work we have studied some of the enzymatic properties of three N-acetyl-β-D-hexosaminidases encoded by the genome of the intestinal isolate Bifidobacterium longum subsp. infantis ATCC 15697 and the gene expression of the corresponding genes during bacterial growth on human milk oligosaccharides. These enzymes belong to the glycosyl hydrolase family 20, with several homologs in bifidobacteria. Their optimum pH was 5.0 and optimum temperature was 37 °C. The three enzymes were active on the GlcNAcβ1-3 linkage found in lacto-N-tetraose, the most abundant human milk oligosaccharide. Blon_0459 and Blon_0732, but not Blon_2355, cleaved branched GlcNAcβ1-6 linkages found in lacto-N-hexaose, another oligosaccharide abundant in breast milk. Bifidobacterium infantis N-acetyl-β-D-hexosaminidases were induced during early growth in vitro on human milk oligosaccharides, and also during growth on l...Continue Reading

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Citations

Oct 9, 2012·Applied and Environmental Microbiology·Diego Muñoz-ProvencioVicente Monedero
May 16, 2012·Advances in Nutrition·Daniel GarridoDavid A Mills
May 23, 2014·Annual Review of Nutrition·Jennifer T SmilowitzSamara L Freeman
Sep 10, 2013·Applied and Environmental Microbiology·Frederik De BruynMarjan De Mey
May 29, 2015·Biotechnology, Biotechnological Equipment·Velichka PavlovaElena Nikolova
Nov 12, 2014·Annual Review of Animal Biosciences·Alline R PachecoDavid A Mills
Dec 31, 2015·Nutrition and Metabolic Insights·Aifric O'SullivanJennifer T Smilowitz
Oct 11, 2014·Pediatric Research·Mark A UnderwoodDavid A Mills
Jul 31, 2013·Applied and Environmental Microbiology·Santiago Ruiz-MoyanoDavid A Mills
Apr 17, 2016·Applied and Environmental Microbiology·Sercan KaravDavid A Mills
Dec 22, 2019·Microorganisms·Korin AlbertDavid A Sela
Aug 18, 2017·Bioscience, Biotechnology, and Biochemistry·Toshihiko KatohTakane Katayama
Aug 16, 2019·Gastroenterology Report·Hu HaoHoward S Faden
Jan 10, 2016·American Journal of Physiology. Lung Cellular and Molecular Physiology·Stavros GarantziotisLorenzo Drago
Sep 5, 2018·Frontiers in Microbiology·Manuel ZúñigaMaría J Yebra
Mar 28, 2018·Annual Review of Food Science and Technology·Nina KirmizDavid A Mills
Jul 18, 2015·Microbiology Spectrum·Alline R Pacheco, Vanessa Sperandio
Sep 2, 2021·Applied and Environmental Microbiology·Gerald W Tannock

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