Release from endoplasmic reticulum matrix proteins controls cell surface transport of MHC class I molecules

Traffic
Susanne FritzscheSebastian Springer

Abstract

The anterograde transport of secretory proteins from the endoplasmic reticulum (ER) to the plasma membrane is a multi-step process. Secretory proteins differ greatly in their transport rates to the cell surface, but the contribution of each individual step to this difference is poorly understood. Transport rates may be determined by protein folding, chaperone association in the ER, access to ER exit sites (ERES) and retrieval from the ER-Golgi intermediate compartment or the cis-Golgi to the ER. We have used a combination of folding and trafficking assays to identify the differential step in the cell surface transport of two natural allotypes of the murine major histocompatibility complex (MHC) class I peptide receptor, H-2D(b) and H-2K(b) . We find that a novel pre-ER exit process that acts on the folded lumenal part of MHC class I molecules and that drastically limits their access to ERES accounts for the transport difference of the two allotypes. Our observations support a model in which the cell surface transport of MHC class I molecules and other type I transmembrane proteins is governed by the affinity of all their folding and maturation states to the proteins of the ER matrix.

References

Jul 27, 1990·Cell·A TownsendA Tse
Aug 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·G J HämmerlingU Hämmerling
Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·E M Croze, D J Morré
Feb 1, 1996·Journal of Molecular Graphics·W HumphreyK Schulten
Feb 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·J L Campbell, R Schekman
May 12, 2000·Nature Cell Biology·S NehlsJ Lippincott-Schwartz
Mar 23, 2001·Nature Immunology·N GarbiG J Hämmerling
Apr 6, 2002·International Immunology·Marie-Eve Paquet, David B Williams
Apr 24, 2002·Immunity·Anthony P WilliamsTim Elliott
Jun 10, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marie-Eve PaquetDavid B Williams
Aug 10, 2007·Seminars in Cell & Developmental Biology·Marcus C S Lee, Elizabeth A Miller
Oct 24, 2009·FEBS Letters·Annika Budnik, David J Stephens
Apr 19, 2011·Annual Review of Biochemistry·Ineke Braakman, Neil J Bulleid
Jul 9, 2011·Tissue Antigens·P M Saunders, P van Endert
Aug 13, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Malgorzata Anna GarstkaSebastian Springer
Jan 10, 2013·Annual Review of Immunology·Janice S BlumPeter Cresswell
Feb 20, 2013·Molecular Immunology·Susanne Fritzsche, Sebastian Springer

❮ Previous
Next ❯

Citations

Mar 17, 2015·Current Opinion in Immunology·Sebastian Springer
Feb 1, 2020·Annual Review of Biophysics·Simon Trowitzsch, Robert Tampé

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.