Release kinetics of fluorescent dyes from PLGA nanoparticles in retinal blood vessels: in vivo monitoring and ex vivo localization

European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V
Enqi ZhangPetra Henrich-Noack

Abstract

PLGA (poly(lactic-co-glycolic acid))-based nanoparticles (NPs) are promising carrier-cargo systems because of their excellent biocompatibility and possibility of sustained drug release. However, it is not well understood how the kinetics of such drug delivery system perform in the retinal blood circulation as imaged in vivo and in real time. To answer this question, PLGA NPs were loaded either with lipophilic carbocyanine perchlorate (DiI) or hydrophilic Rhodamine 123 (Rho123) and coated with poloxamer 188 (P188): PLGA-DiI/P188 and PLGA-Rho123/P188. All particles had narrow size distributions around 130 nm, spherical shape and negative potential. Subsequently, we performed in vivo real-time imaging of retinal blood vessels, combined with ex vivo microscopy to monitor the kinetics and to detect location of those two fluorescent markers. We found that DiI signals were long lasting, detectable longer than 90 min in blood vessels after intravenous injection as visible by homogeneous labelling of the vessel wall as well as by spots in the lumen of blood vessels. In contrast, Rho123 signals mostly disappeared after 15 min post intravenous injection in such compartment. To explore how PLGA NP-loaded cargoes are released in vivo in the...Continue Reading

Citations

Jun 12, 2021·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Enqi ZhangPetra Henrich-Noack
Sep 6, 2021·International Journal of Pharmaceutics·Harshvardhan ModhMatthias G Wacker

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