Release kinetics of intact and degraded troponin I and T after irreversible cell damage

Experimental and Molecular Pathology
Marleen HesselA van der Laarse

Abstract

We characterized the release kinetics of cardiac troponin I and T in relation to lactate dehydrogenase (LDH) from cardiomyocytes before and after the transition from reversible to irreversible cell damage. Cardiomyocytes were exposed to mild metabolic inhibition (1 mmol/L sodium azide) to induce a necrotic cell death process that is characterized by a reversible (0-12 h) and irreversible phase (12-30 h). At various time intervals cells and media were collected and analyzed for LDH activity, intact cTnI and cTnT, and their degradation products. During the first 12 h of metabolic inhibition, cell viability was unchanged with no release of intact cTnI and cTnT nor their degradation products. Between 12 and 30 h of azide treatment, cardiomyocytes showed progressive cell death accompanied by release of intact cTnI (29 kDa), intact cTnT (39 kDa), four cTnI degradation products of 26, 20, 17 and 12 kDa, and three cTnT degradation products of 37, 27 and 14 kDa. Possibly due to degradation, there is progressive loss of cTnI and cTnT protein that is obviously undetected by the antibodies used. Metabolic inhibition of cardiomyocytes induces a parallel release of intact cTnI and cTnT and their degradation products, starting only after onse...Continue Reading

References

Oct 1, 1990·Journal of Molecular and Cellular Cardiology·M Persoon-RothertA van der Laarse
Dec 1, 1989·Journal of Molecular and Cellular Cardiology·H A KatusW Kübler
Jul 21, 1998·Journal of the American College of Cardiology·C A PolanczykP A Johnson
Oct 8, 1998·Biochemistry and Molecular Biology International·V L FilatovN B Gusev
Mar 30, 2001·Biotechnology and Applied Biochemistry·N MorjanaR Tal
Dec 18, 2001·International Journal of Cardiology·J P NunesD B Faria
Jul 12, 2002·Pflügers Archiv : European journal of physiology·M BoermaL H F Mullenders
Dec 17, 2002·Molecular and Cellular Biochemistry·Marion Persoon-RothertArnoud van der Laarse
Oct 1, 1955·Proceedings of the Society for Experimental Biology and Medicine·F WROBLEWSKI, J S LADUE
Dec 24, 2003·Circulation·Jart H C DirisMarja P van Dieijen-Visser
Feb 10, 2004·Pflügers Archiv : European journal of physiology·Lijuan LiArnoud van der Laarse
Dec 23, 2004·Clinical Chemistry·Etienne C H J MichielsenMarja P Van Dieijen-Visser
Dec 14, 2006·Clinical Chemistry and Laboratory Medicine : CCLM·Etienne C H J MichielsenMarja P Van Dieijen-Visser
May 22, 2007·Clinical Biochemistry·Etienne C H J MichielsenMarja P Van Dieijen-Visser

❮ Previous
Next ❯

Citations

Mar 13, 2014·Experimental and Molecular Pathology·Alexander S StrengMarja P van Dieijen-Visser
Dec 3, 2014·The American Journal of Cardiology·Sean P CollinsMihai Gheorghiade
Aug 6, 2010·European Heart Journal·Kristian ThygesenUNKNOWN Study Group on Biomarkers in Cardiology of the ESC Working Group on Acute Cardiac Care
Jan 24, 2012·European Heart Journal·Raphael TwerenboldChristian Mueller
Aug 10, 2010·Analytical and Bioanalytical Chemistry·Henrik AnderssonCarl-Fredrik Mandenius
Oct 29, 2014·Acta Anaesthesiologica Scandinavica·P Juhl-OlsenE Sloth
Apr 11, 2009·Journal of the American College of Cardiology·Arnold Koller, Wolfgang Schobersberger
Jul 23, 2013·Journal of Molecular and Cellular Cardiology·Alexander S StrengWill K W H Wodzig
Sep 15, 2014·Clinica Chimica Acta; International Journal of Clinical Chemistry·Claudio PassinoAldo Clerico
Oct 11, 2017·Cardiovascular Research·Kyung Chan ParkMichael S Marber
Nov 9, 2017·BMC Veterinary Research·Persephone Greco-OttoRenaud Léguillette
Dec 28, 2017·European Heart Journal. Acute Cardiovascular Care·Johannes MairAllan S Jaffe
Jan 24, 2015·The European Respiratory Journal·Anja KaeberichMareike Lankeit
Aug 28, 2014·Current Opinion in Critical Care·Danielle M GualandroChristian Mueller
Aug 30, 2017·Molecular Medicine Reports·Xuehua XuShaohua Zhu
Oct 29, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Minghui LiXiaojun Han
Jan 17, 2021·International Journal of Molecular Sciences·Birte WeberMiriam Kalbitz
May 27, 2020·Revista Española De Cardiología·María Rubini GiménezChristian Mueller
Aug 8, 2020·Hematology/oncology and Stem Cell Therapy·Salem E DerazAsmaa A Mahmoud

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.