Release of anti-inflammatory peptides from thermosensitive nanoparticles with degradable cross-links suppresses pro-inflammatory cytokine production

Biomacromolecules
Scott PohAlyssa Panitch

Abstract

Pro-inflammatory cytokines tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6) are mediators in the development of many inflammatory diseases. To demonstrate that macrophages take up and respond to thermosensitive nanoparticle drug carriers, we synthesized PEGylated poly(N-isopropylacrylamide-2-acrylamido-2-methyl-1-propanesulfonate) particles cross-linked with degradable disulfide (N,N'-bis(acryloyl)cystamine) (NGPEGSS). An anti-inflammatory peptide (KAFAK) was loaded and released from the thermosensitive nanoparticles and shown to suppress levels of TNF-α and IL-6 production in macrophages. Cellular uptake of fluorescent, thermosensitive, and degradable nanoparticles and therapeutic efficacy of free KAFAK peptide compared to that of KAFAK loaded in PEGylated degradable thermosensitive nanoparticles were examined. The data suggests that the degradable, thermosensitive nanoparticles loaded with KAFAK may be an effective tool to treat inflammatory diseases.

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Citations

Jun 1, 2016·Nanomedicine : Nanotechnology, Biology, and Medicine·Jenny B LinAlyssa Panitch
Dec 2, 2017·Chemistry : a European Journal·Cristina de la TorreFélix Sancenón
Jul 23, 2020·Advanced Drug Delivery Reviews·Romain BrusiniPatrick Couvreur
Mar 13, 2021·Biomedical Materials·Xueli MeiElizabeth R Gillies
Apr 17, 2021·Drug Delivery and Translational Research·Zhengyu Deng, Shiyong Liu

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