Release of cell-free DNA into the bloodstream leads to high levels of non-tumor plasma DNA during tumor progression in rats

Cancer Letters
Dolores C García-OlmoDamián García-Olmo

Abstract

To examine the implications of cell-free DNA in the plasma in neoplastic disease, it is necessary to clarify various features of this DNA, such as the contribution of DNA from the host's normal cells and the kinetics of the release of this latter DNA. To quantify non-tumor DNA in the plasma of tumor-bearing rats and to correlate levels of this DNA with tumor progression, we injected DHD/K12-PROb colon cancer cells subcutaneously into BD-IX rats and recorded tumor diameters weekly. After euthanasia, we collected plasma from each rat and quantified non-mutated and mutated DNA in the plasma. Overall, levels of non-mutated (non-tumor) DNA in plasma of tumor-bearing animals were significantly higher than those in healthy animals measured by real-time PCR (p=0.001). However, 5 weeks after inoculation, levels were similar to those in healthy animals. As a whole, levels of non-mutated DNA were not significantly related to tumor size or to metastasis. However, when we excluded animals that were analyzed earliest, we found a positive and statistically significant correlation between levels of non-mutated DNA and tumor diameter (p=0.002). Release of cell-free DNA into the plasma during tumor progression appears to follow a predictable tim...Continue Reading

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Citations

Feb 5, 2013·Molecular Cancer·Dolores C García-OlmoDamián García-Olmo
Aug 29, 2012·Journal of Cancer Research and Clinical Oncology·Chanida VinayanuwattikunVirote Sriuranpong
May 24, 2012·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Tobias M GorgesOliver von Ahsen
Aug 7, 2010·Clinica Chimica Acta; International Journal of Clinical Chemistry·Klaus JungAnja Rabien
Nov 17, 2009·Enfermería clínica·Paloma de la Quintana Jiménez
Sep 10, 2015·Biomedit︠s︡inskai︠a︡ khimii︠a︡·O E Bryzgunova, P P Laktionov
Jun 1, 2014·Critical Reviews in Clinical Laboratory Sciences·Bernhard RallaKlaus Jung
Jun 22, 2018·Oncotarget·Dipesh Kumar YadavTingbo Liang
Jan 27, 2017·Biomedical Reports·Shankar SurajSweta Srivastava
Jan 19, 2018·Expert Reviews in Molecular Medicine·Peter CelecPeter Boor

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