Release of extraction-resistant mRNA in stationary phase Saccharomyces cerevisiae produces a massive increase in transcript abundance in response to stress

Genome Biology
Anthony D AragonMargaret Werner-Washburne

Abstract

As carbon sources are exhausted, Saccharomyces cerevisiae cells exhibit reduced metabolic activity and cultures enter the stationary phase. We asked whether cells in stationary phase cultures respond to additional stress at the level of transcript abundance. Microarrays were used to quantify changes in transcript abundance in cells from stationary phase cultures in response to stress. More than 800 mRNAs increased in abundance by one minute after oxidative stress. A significant number of these mRNAs encode proteins involved in stress responses. We tested whether mRNA increases were due to new transcription, rapid poly-adenylation of message (which would not be detected by microarrays), or potential release of mature mRNA present in the cell but resistant to extraction during RNA isolation. Examination of the response to oxidative stress in an RNA polymerase II mutant, rpb1-1, suggested that new transcription was not required. Quantitative RT-PCR analysis of a subset of these transcripts further suggested that the transcripts present in isolated total RNA from stationary phase cultures were polyadenylated. In contrast, over 2,000 transcripts increased after protease treatment of cell-free lysates from stationary phase but not ex...Continue Reading

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Citations

Jan 18, 2008·Molecular Biology of the Cell·Anthony D AragonMargaret Werner-Washburne
Feb 4, 2011·Molecular Biology of the Cell·George S DavidsonMargaret Werner-Washburne
May 4, 2010·Eukaryotic Cell·Ari RosenbachCarol A Kumamoto
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Sep 12, 2012·Genetics·James R Broach
Jan 1, 2011·Genetics Research International·José E Pérez-OrtínAntonio Jordán-Pla
Sep 9, 2008·Molecular and Biochemical Parasitology·Anthony D AragonCharles Cunningham
Jul 2, 2008·Nature Reviews. Genetics·Luis López-MauryJürg Bähler
May 6, 2019·Current Genetics·Sarah G Swygert, Toshio Tsukiyama
Oct 2, 2014·Archives of Toxicology·Mirjam M SchaapMirjam Luijten
Mar 5, 2011·Molecular Microbiology·Xavier EscotéJoan Vendrell
Dec 29, 2020·Nucleic Acids Research·Po-Hsuen Lee, Mary Ann Osley
Mar 11, 2008·Biochimica Et Biophysica Acta·Choon Pei Low, Hongyuan Yang
Sep 5, 2008·Archives of Biochemistry and Biophysics·Hanna GalganskaHanna Kmita

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Datasets Mentioned

BETA
GSE3729

Methods Mentioned

BETA
environmental stress
Precipitation
electrophoresis

Software Mentioned

GenePix Pro
ABI Prism 7000 SDS
SOM

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