PMID: 6412967Aug 1, 1983Paper

Release of immunoreactive TRH in serum-free cultures of mouse hypothalamic cells

Brain Research
C LoudesA Tixier-Vidal

Abstract

Serum-free cultures of mouse hypothalamic cells were used as a model for studying TRH (thyroliberin) secretion in vitro. Supplementation of the culture medium with triiodothyronine, corticosterone and polyunsaturated fatty acids is necessary to obtain a substantial release capacity of TRH neurons. Under these conditions depolarization of the cells with 60 mM K+ results in a calcium-dependent release of immunoreactive TRH.

References

Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J E Bottenstein, G H Sato
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R GamseS E Leeman
May 13, 1982·Brain Research·R A Peterfreund, W Vale
Jan 1, 1981·Developmental Neuroscience·A Faivre-BaumanA Tixier-Vidal

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Citations

Mar 1, 1990·Brain Research. Developmental Brain Research·M SebbenA Dumuis
Oct 27, 1995·Brain Research. Developmental Brain Research·J L CharliP Joseph-Bravo
Jan 1, 1989·Neurochemistry International·C LoudesA Faivre-Bauman
Sep 15, 2001·Brain Research. Developmental Brain Research·L Pérez-MartínezP Joseph-Bravo
May 30, 2002·Brain Research. Brain Research Protocols·Patricia Joseph-BravoJean-Louis Charli
Sep 13, 2001·The European Journal of Neuroscience·M Guerra-CrespoL Pérez-Martínez
Sep 22, 2009·Brain Research·Alfonso Carreón-RodríguezLeonor Pérez-Martínez
Aug 1, 1992·Molecular and Cellular Neurosciences·Y KagotaniA Tixier-Vidal
Jan 1, 1993·Prostaglandins, Leukotrienes, and Essential Fatty Acids·J M BourreM Piciotti

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