Release of interleukin-6 via the regulated secretory pathway in PC12 cells

Neuroscience Letters
Jens Carsten MöllerEric M Shooter

Abstract

A growing body of evidence suggests that diverse growth factors such as neurotrophins (NTs), insulin-like growth factor-1 (IGF-1), and glial cell line-derived neurotrophic factor (GDNF) can be released via the regulated secretory pathway in neuronal cells, possibly representing a mechanism for preferentially supplying these growth factors to active synapses. Here we investigated whether interleukin-6 (IL-6), a member of the family of neuropoietic cytokines, can be released via stimulus-coupled secretion as well. IL-6 was expressed in PC12 cells, a neuronal model cell line that is frequently used for the study of vesicle release and trafficking. Regulated secretion of this cytokine was induced by 0.5 mM ATP and treatment with epidermal growth factor (EGF) and nerve growth factor (NGF). Release induced by 0.5 mM ATP but not by NGF or EGF depended on the presence of extracellular Ca(++). Furthermore, IL-6 colocalized with the dense core vesicle (DCV)-marker secretogranin-II (Sg-II) in transfected PC12 cells. Our data suggest that the neuropoietic cytokine IL-6 can be sorted to the regulated secretory pathway in neuronal cells and indicate a potential role for this cytokine in synaptic plasticity.

References

Sep 4, 1987·Science·R Levi-Montalcini
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·I L CampbellL Mucke
Nov 1, 1993·The European Journal of Neuroscience·B SchöbitzF Holsboer
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M A Castro-Alamancos, I Torres-Aleman
Aug 30, 1996·The Journal of Biological Chemistry·A Blöchl, C Sirrenberg
Oct 11, 1996·The Journal of Biological Chemistry·J V HeymachE M Shooter
Jun 1, 1996·Molecular and Cellular Neurosciences·W D Snider, J W Lichtman
Feb 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·C J HeyserL H Gold
Jan 1, 1997·Progress in Neurobiology·M YamadaH Hatanaka
Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·M CanossaH Thoenen
Mar 26, 1998·Journal of Neuroscience Research·J C MöllerE M Shooter
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·A KrüttgenE M Shooter
Dec 10, 1999·Molecular and Cellular Neurosciences·J L Jankowsky, P H Patterson
Jun 14, 2000·Current Opinion in Neurobiology·D R Kaplan, F D Miller
Mar 18, 2003·The Journal of Biological Chemistry·Mohamed T GhorbelDavid Murphy
Sep 4, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D BalschunH O Besedovsky

❮ Previous
Next ❯

Citations

Oct 8, 2009·Circulation. Heart Failure·Hector R MobineElazer R Edelman
Oct 16, 2007·Molecular and Cellular Neurosciences·Niki TsakiriEmmanuel Pinteaux
Feb 25, 2009·Annals of the New York Academy of Sciences·Elizabeth Giestal de AraujoAline Araujo Dos Santos
Nov 19, 2009·Journal of Neurochemistry·Jonathan VinetKnut Biber
Jun 13, 2009·Epilepsy Research·Sebastian BauerHajo M Hamer
Nov 19, 2010·Journal of Hepatology·Jonathan BrückDieter Häussinger
Aug 14, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Eiko K de JongKnut Biber
Jul 18, 2018·Frontiers in Endocrinology·Collin J ByrneT C Tai

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.