Abstract
To further investigate the mechanisms accounting for defective natural killer (NK) activity observed in the majority of patients with lymphoproliferative disease of granular lymphocytes (LDGL), we have studied the generation of natural killer cytotoxic factor (NKCF) from peripheral blood lymphocytes recovered from twelve LDGL patients. On the basis of their cytotoxic activity against the K-562 target cells, cases under study were separated in two groups. Cells from five patients, referred to as NK+, were found to exhibit high levels of NK-cell mediated cytotoxicity; cells from seven patients, referred to as NK-, despite their ability to bind to the K-562 targets, displayed a defective NK function. The coculture of cells from NK+ patients with NK sensitive K-562 cells triggered a significantly higher production of NKCF with respect to NK- patients (p less than 0.001 at 1:2 dilution). Using phytohemagglutinin (PHA) as the NKCF stimulator, differences between the groups were not significant, due to a recovery of NKCF generation in NK- patients. Furthermore, a consistent lectin (PHA) dependent cellular cytotoxicity (LDCC) was exhibited by both groups of patients. The addition of gamma-interferon (IFN-gamma) or interleukin-2 (IL-2) ...Continue Reading
References
Jul 1, 1978·Cellular Immunology·G A GrangerJ C Hiserodt
Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·E A CarswellB Williamson
Jun 1, 1986·Archives of Internal Medicine·W C ChanT A Waldmann
Jan 15, 1986·International Journal of Cancer. Journal International Du Cancer·P AllavenaA Mantovani
Sep 1, 1987·Cellular Immunology·K ItohC M Balch
May 1, 1987·Journal of Leukocyte Biology·H KorenN Ruddle
Sep 1, 1987·Medicine·T P Loughran, G Starkebaum
Dec 15, 1987·Cancer·G SemenzatoM Vespignani
Nov 1, 1985·The Journal of Experimental Medicine·G DegliantoniG Trinchieri
Jul 1, 1984·Journal of Clinical Immunology·A LandayC E Grossi
Aug 12, 1983·Journal of Immunological Methods·M Vargas-CortesP Perlmann
Apr 1, 1983·Cellular Immunology·M L WeitzenG A Granger
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·C M ZacharchukH S Shin
Jan 1, 1982·Journal of Immunological Methods·T TimonenR B Herberman
Nov 1, 1980·Infection and Immunity·D N MännelS E Mergenhagen
Oct 1, 1982·Infection and Immunity·J Nissen-Meyer, J Hammerstrøm